High-force magnetic tweezers with force feedback for biological applications
High-force magnetic tweezers with force feedback for biological applications
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DOI：
10.1063/1.2804771
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发表时间：
2007-11-01
影响因子：
1.6
通讯作者：
Fabry, Ben
中科院分区：
文献类型：
--
作者：
Kollmannsberger, Philip;Fabry, Ben
使用磁性镊子的磁性微操作是一种通用的生物物理技术，已被用于单分子展开、流变学测量和活细胞内力调节过程的研究。本文描述了一种廉价的磁镊子装置，用于在5微米磁珠上施加高达100nN的精确控制力。采用参数化力标定方法，通过实时控制磁镊子的位置和电流，实现了力的高精度。磁珠与磁珠之间的距离只有几微米，就能获得高的作用力。施加如此大的力可以用来表征软材料在非线性区域的局部粘弹性，或者研究力调节过程和活细胞中的机械力化学信号转导。该装置可以很容易地适用于任何倒置显微镜。(C)2007年美国物理研究所。
Magnetic micromanipulation using magnetic tweezers is a versatile biophysical technique and has been used for single-molecule unfolding, rheology measurements, and studies of force-regulated processes in living cells. This article describes an inexpensive magnetic tweezer setup for the application of precisely controlled forces up to 100 nN onto 5 mu m magnetic beads. High precision of the force is achieved by a parametric force calibration method together with a real-time control of the magnetic tweezer position and current. High forces are achieved by bead-magnet distances of only a few micrometers. Applying such high forces can be used to characterize the local viscoelasticity of soft materials in the nonlinear regime, or to study force-regulated processes and mechanochemical signal transduction in living cells. The setup can be easily adapted to any inverted microscope. (c) 2007 American Institute of Physics.