Fibronectin biosynthesis in the rat aorta in vitro. Changes due to experimental hypertension.
Fibronectin biosynthesis in the rat aorta in vitro. Changes due to experimental hypertension.
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体外大鼠主动脉中的纤连蛋白生物合成。
DOI：
10.1172/jci115420
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Brecher,P
中科院分区：
文献类型：
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作者：
Saouaf,R;Takasaki,I;Eastman,E;Chobanian,AV;Brecher,P
本研究旨在确定纤连蛋白生物合成的变化是否伴随实验性高血压后主动脉组织中发生的表型变化。开发了一种体外程序来测量从正常血压或高血压大鼠获得的主动脉环中的纤连蛋白合成。用脱氧皮质酮/盐处理7天和21天的大鼠主动脉环的纤连蛋白生物合成增加了三到六倍，这种变化在21天时更加明显。相比之下，在任一时间点，总蛋白的净掺入量均没有重大变化。对 10 至 40 周龄 Wistar 大鼠和自发性高血压大鼠主动脉环中纤连蛋白生物合成进行比较的研究表明，两种品系的老年动物中纤连蛋白生物合成均有所增加，但品系之间仅有轻微差异。使用注射血管紧张素 II 的大鼠进行的研究表明，血压升高与主动脉纤连蛋白生物合成增加之间存在相关性。主动脉提取物的蛋白质印迹分析表明，高血压模型中纤连蛋白含量增加。用于测量纤连蛋白生物合成的体外程序似乎可以可靠地反映体内纤连蛋白表达的变化，并且该方法可用于研究影响血管组织中蛋白质表达的因素。
This study was undertaken to determine if changes in fibronectin biosynthesis accompany the phenotypic changes that occur in aortic tissue following experimental hypertension. An in vitro procedure was developed to measure fibronectin synthesis in aortic rings obtained from normotensive or hypertensive rats. There was a three to sixfold increase in fibronectin biosynthesis by aortic rings taken from rats treated with deoxycorticosterone/salt for 7 and 21 d, the change being more pronounced at 21 d. In contrast, there was no major change at either time point in net incorporation into total protein. Studies comparing fibronectin biosynthesis in aortic rings from Wistar rats and spontaneously hypertensive rats at ages between 10 and 40 wk showed increased fibronectin biosynthesis in older animals of both strains, but only slight differences between strains. Studies using rats infused with angiotensin II showed a correlation between blood pressure elevation and increased aortic fibronectin biosynthesis. Western blot analysis of aortic extracts showed that the fibronectin content was increased in the hypertensive models. The in vitro procedure for measuring fibronectin biosynthesis appears to provide a reliable reflection of in vivo changes in fibronectin expression, and the methodology could prove useful for studying the factors influencing protein expression in vascular tissue.Images