MATRIX-ASSISTED LASER DESORPTION WITH ULTRA-SHORT LASER-PULSES
MATRIX-ASSISTED LASER DESORPTION WITH ULTRA-SHORT LASER-PULSES
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DOI：
10.1002/rcm.1290060307
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发表时间：
1992-03-01
影响因子：
2
通讯作者：
SIEGBAHN, K
中科院分区：
文献类型：
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作者：
DEMIREV, P;WESTMAN, A;SIEGBAHN, K
准分子泵浦的染料激光器在紫外（UV，248 nm）和可见（496 nm）光谱范围内产生超短（560 fs半高全宽，FWHM）脉冲，用于从不同基质中解吸蛋白质离子。 本文报道了激光能量和功率密度对胰岛素二次离子产额的影响。 将这些数据与采用氮激光器（337 nm，3 ns FWHM）获得的数据进行比较。 建立了超短脉冲基质辅助激光解吸（MALD）胰岛素离子的阈值能量密度。 对于所采用的两个激光系统，该阈值能量具有相同的数量级，并且不依赖于激光脉冲长度（能量沉积时间）。 因此，我们证明了沉积到样品中的激光能量的量，而不是激光功率，是MALD过程中的重要参数。
An excimer pumped dye laser generating ultra-short (560 fs full width at half maximum, FWHM) pulses in the ultraviolet (UV, 248 nm) and visible (496 nm) spectral ranges is used to desorb protein ions from different matrices. The effects of laser-light energy and power density on secondary-ion yields from insulin are reported. These data are compared to data obtained by employing a nitrogen laser (337 nm, 3 ns FWHM). The existence of a threshold energy density for the matrix-assisted laser desorbtion (MALD) of insulin ions by the ultra-short pulses is established. This threshold energy is of the same order of magnitude for the two laser systems employed and does not depend on the laser pulse length (the energy deposition time). Thus we demonstrate that the amount of laser energy deposited into the sample, rather than the laser power, is the important parameter in the MALD process.