Immunoreactivity of Antibodies Raised Against Synthetic Peptide Fragments Predicted from Cysteine-rich and Carboxy-terminal Domains of Dystrophin cDNA
Immunoreactivity of Antibodies Raised Against Synthetic Peptide Fragments Predicted from Cysteine-rich and Carboxy-terminal Domains of Dystrophin cDNA
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根据肌营养不良蛋白 cDNA 的富含半胱氨酸和羧基末端结构域预测的针对合成肽片段的抗体的免疫反应性
DOI：
10.15369/sujms1989.4.141
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
S. Miyake
中科院分区：
文献类型：
--
作者：
T. Jimi;Y. Wakayama;A. Takeda;N. Misugi;T. Kumagai;S. Miyake
我们合成了6个富含半胱氨酸和羧基（C）-末端结构域的肽片段，从肌营养不良蛋白cDNA图谱预测，并使用6种针对这些合成肽的抗体对62块活检肌肉进行了免疫化学研究。22例正常对照和16例Duchenne型肌营养不良症（DMD）和Becker型肌营养不良症（BMD）以外的疾病患者明确显示肌纤维表面膜中的肌营养不良蛋白连续染色的五种抗体，而11例DMD患者呈阴性染色。13例BMD患者中有7例显示斑片状和微弱的免疫标记，但其他BMD患者的免疫染色为阴性。大多数BMD患者在外显子45-47或45-48有缺失位点。使用6种抗体对正常肌肉进行免疫印迹，得到400 kDa的单一条带，而在DMD患者中没有观察到条带。在C-末端抗体对照中观察到额外的320 kDa条带。
We synthesized six peptide fragments of cysteine-rich and Carboxy (C)-terminal domains predicted from the dystrophin cDNA map, and immunohistochemically studied 62 biopsied muscles using six antibodies against these synthetic peptides. Twenty-two normal controls and 16 patients with diseases other than Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) clearly revealed continuous staining of dystrophin in the myofiber surface membrane by five antibodies, whereas 11 DMD patients showed negative staining. Seven of 13 BMD patients revealed patchy and faint immunolabelling, but immunostaining was negative for the other BMD patients. Most BMD patients had deletion sites at exons 45-47 or 45-48. Immunoblotting of normal muscles using six antibodies gave a 400 kDa single band, whereas no band was observed in DMD patients. An additional 320 kDa band was noted in controls with C-terminal antibodies.
影响因子：
4.4
作者：
Monaco, Anthony P.;Bertelson, Corlee J.;Kunkel, Louis M.
通讯作者：
Kunkel, Louis M.