Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections.
Immunohistochemical techniques for detection of dermatan sulfate proteoglycan in tissue sections.
复制标题
用于检测组织切片中硫酸皮肤素蛋白多糖的免疫组织化学技术。
DOI：
10.3109/10520298909108042
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Yoshida
中科院分区：
文献类型：
--
作者：
M. Sobue;J. Takeuchi;T. Fukatsu;T. Nagasaka;N. Nakashima;T. Ogura;T. Katoh;K. Yoshida
在用软骨素B-裂解酶（0.01单位/ml）在20 mM Tris-HCl（pH 8.0）中处理1小时，然后用偶联N-乙酰半乳糖胺-4硫酸盐的对不饱和糖醛酸特异的抗体9A 2染色的组织切片中检测硫酸皮肤素蛋白聚糖链。相反，用软骨素B-裂解酶处理后，用分别与偶联N-乙酰半乳糖胺6-硫酸盐的不饱和糖醛酸和偶联N-乙酰半乳糖胺的不饱和糖醛酸反应的抗体3B 3和1B 5未观察到阳性染色。由此揭示的硫酸皮肤素的分布通过与单克隆抗体6 B6所发现的分布进行比较来证实，所述单克隆抗体6 B6与携带硫酸皮肤素侧链的小蛋白聚糖反应。两种方法在纤维结缔组织中的阳性染色定位几乎一致。
Dermatan sulfate proteoglycan chains were detected in tissue sections treated with chondroitin B-lyase (0.01 units/ml) in 20 mM Tris-HCl (pH 8.0) for 1 hr, followed by staining with antibody 9A2 specific for unsaturated uronic acid coupled to N-acetylgalactosamine-4 sulfate. In contrast, after treatment with chondroitin B-lyase, no positive staining was observed with antibodies 3B3 and 1B5 which react to the unsaturated uronic acid coupled to N-acetylgalactosamine 6-sulfate and unsaturated uronic acid coupled to N-acetylgalactosamine, respectively. The distribution of dermatan sulfate thus revealed was confirmed by comparison with that found by monoclonal antibody 6B6 which reacts with small proteoglycans carrying dermatan sulfate side chains. The localization of positive staining in fibrous connective tissues was almost identical with these two procedures.