Eicosapentaenoic acid induces mRNA expression of peroxisome proliferator-activated receptor γ
Eicosapentaenoic acid induces mRNA expression of peroxisome proliferator-activated receptor γ
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DOI：
10.1038/oby.2002.70
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发表时间：
2002-06-01
期刊：
影响因子：
--
通讯作者：
Vidal, H
中科院分区：
文献类型：
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作者：
Chambrier, C;Bastard, JP;Vidal, H
目的：为了验证是否多不饱和脂肪酸（PUFAs）可以调节核受体过氧化物酶体增殖物激活受体γ（PPARgamma）在人类脂肪组织中的表达，研究方法和程序：各种PUFAS对PPARgamma 1和-gamma 2 mRNA表达的影响进行了研究，在新鲜分离的脂肪细胞从手术过程中获得的脂肪样品制备。还测定了处于禁食状态的II型肥胖妇女的皮下脂肪组织活组织检查中的PPARgamma mRNA水平，以寻找PPARgamma表达和血浆PUFA浓度之间的体内关联。结果：二十碳五烯酸（EPA）可显著增加脂肪细胞中PPARgamma 1的mRNA水平，但不影响PPARgamma 2的表达。其他测试的脂肪酸（亚麻酸、二十二碳六烯酸和w-6 PUFA）没有影响。EPA的作用依赖于浓度（50 μ M 6小时后的最大作用），并且不能被PPAR家族的不同成员的激活剂复制。此外，血浆EPA浓度和PPARgamma mRNA水平在脂肪组织中的肥胖subjects.Discussion：我们的研究结果表明，脂肪组织PPARgamma 1 mRNA的浓度正调控EPA，这表明，饮食脂质的组成可能会影响PPARgamma基因在体内的表达在人类。这些数据还表明，诱导这种核受体亚型的表达可能涉及EPA的作用机制及其某些有益作用。
Objective: To verify whether polyunsaturated fatty acids (PUFAs) can regulate the expression of the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) in human adipose tissue.Research Methods and Procedures: The effect of various PUFAS on PPARgamma1 and -gamma2 mRNA expression was investigated in freshly isolated adipocytes prepared from fat samples obtained during surgery. PPARgamma mRNA levels were also determined in subcutaneous adipose tissue biopsies of I I obese women, in the fasting state, to search for in vivo associations between PPARgamma expression and plasma PUFA concentrations. PPARgamma mRNA levels were determined by reverse-transcription competitive polymerase chain reaction.Results: Eicosapentaenoic acid (EPA) significantly increased PPARgamma1 mRNA levels in isolated adipocytes, with-out affecting the expression of PPARgamma2. The other tested fatty acids (linolenic acid, docosahexaenoic acid and w-6 PUFAs) had no effect. The effect of EPA was dependent on the concentration (maximal effect after 6 hours with 50 muM) and was not reproduced by activators of the different members of the PPAR family. In addition, a strong positive correlation was found between plasma EPA concentrations and PPARgamma mRNA levels in adipose tissue of obese subjects.Discussion: Our results demonstrate that adipose tissue PPARgamma1 mRNA concentration is positively regulated by EPA, suggesting that the composition of dietary lipids may affect PPARgamma gene expression in vivo in humans. These data also suggest that an induction of the expression of this nuclear receptor isoform might be involved in the mechanisin of action of EPA and in some of its beneficial effects.