Quantification of 22 phthalate metabolites in human urine
Quantification of 22 phthalate metabolites in human urine
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DOI：
10.1016/j.jchromb.2007.10.023
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发表时间：
2007-12-01
影响因子：
3
通讯作者：
Calafat, Antonia M.
中科院分区：
文献类型：
--
作者：
Silva, Manori J.;Samandar, Ella;Calafat, Antonia M.
邻苯二甲酸酯是一种普遍存在的工业化学品，人类接触的可能性很大。测量人体中邻苯二甲酸盐代谢物痕量浓度的有效分析方法对于评估邻苯二甲酸盐暴露至关重要。在此之前，我们开发了一种灵敏、准确的自动化分析方法，通过在线固相萃取-同位素稀释-高效液相色谱(HPLC)-电喷雾电离-串联质谱法测量人体尿液中多达16种邻苯二甲酸酯代谢物。包括对另外7种分析物的测量，包括邻苯二甲酸二异ononyl和邻苯二甲酸二异癸酯的氧化代谢物，这两种化学物质广泛用于许多消费品中。我们使用了一种新型的非传统HPLC溶剂梯度程序。通过这种方法，我们在不增加分析运行时间的情况下，对所有22种分析物实现了足够的分辨率和灵敏度，检测限在低ng/mL范围内。该方法具有自动恢复校正、精密度高、样品通量好、基质效应小等优点。虽然有可能以足够的精度和准确度测量这22种邻苯二甲酸盐代谢物，在分份/账单离子水平上，额外的信息，包括毒性动力学数据。需要证明这些邻苯二甲酸酯代谢物对暴露评估的有用性。Elsevier B.V.出版
Phthalates are ubiquitous industrial chemicals with high potential for human exposure. Validated analytical methods to measure trace concentrations of phthalate metabolites in humans are essential for assessing exposure to phthalates. Previously, we developed a sensitive and accurate automated analytical method for measuring up to 16 phthalate metabolites in human urine by using on-line solid phase extraction coupled with isotope dilution-high performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry. To include the measurement of seven additional analytes, including oxidative metabolites of diisononyl and diisodecyl phthalates, two chemicals used extensively in numerous consumer products. we used a novel nontraditional HPLC solvent gradient program. With this approach, we achieved adequate resolution and sensitivity for all 22 analytes with limits of detection in the low ng/mL range, without increasing the analytical run time. The method also has high accuracy with automatic recovery correction, high precision, and excellent sample throughput with minimal matrix effects. Although it is possible to measure these 22 phthalate metabolites with adequate precision and accuracy at sub-parts-per-bill ion levels, additional information, including toxicokinetic data. is needed to demonstrate the usefulness of these phthalate metabolites for exposure assessment purposes. Published by Elsevier B.V.