Usefulness of serum mast cell-specific chymase levels for postmortem diagnosis of anaphylaxis
Usefulness of serum mast cell-specific chymase levels for postmortem diagnosis of anaphylaxis
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DOI：
10.1007/s00414-005-0524-1
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发表时间：
2005-11-01
影响因子：
2.1
通讯作者：
Suzuki, K
中科院分区：
文献类型：
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作者：
Nishio, H;Takai, S;Suzuki, K
食糜酶是一种丝氨酸蛋白酶，主要储存在人类肥大细胞的分泌颗粒中。对 8 例有过敏反应的尸检病例以及 104 例无过敏反应的对照病例进行了血清食糜酶浓度检测。在所有 8 例过敏反应病例中均检测到（范围 3-380 ng/ml，平均 89.8 ng/ml），而在 104 例对照中仅 2 例检测到该蛋白，而在其他 102 例对照中仅检测到 2 例，低于可检测水平（< 3 ng/ml）。已知血清类胰蛋白酶水平是过敏反应的诊断指标，因此在 8 例病例中研究了血清糜蛋白酶和类胰蛋白酶水平之间的关系过敏性死亡；发现显着正相关（r=0.826，p=0.011）。此外，食糜酶在血清中表现出相当稳定。这些结果表明，血清食糜酶水平的测量可能是过敏反应死后诊断的另一个工具。
Chymase, a serine protease, is stored mainly in secretory granules of human mast cells. Serum chymase concentration was examined in 8 autopsy cases with anaphylaxis as well as in 104 control cases without anaphylaxis. It was detected in all 8 cases with anaphylaxis (range 3-380 ng/ml, mean 89.8 ng/ml), while it was detected in only 2 of the 104 controls and was below a detectable level (< 3 ng/ml) in the other 102. Serum tryptase levels are known to be a diagnostic indicator of anaphylaxis, therefore the relationship between serum chymase and tryptase levels was investigated in the 8 cases of anaphylactic death; a significant positive correlation was found (r=0.826, p=0.011). Furthermore, chymase was shown to be quite stable in serum. These results showed that measurement of serum chymase levels might be an additional tool for postmortem diagnosis of anaphylaxis.