Common marmoset embryonic stem cell can differentiate into cardiomyocytes
Common marmoset embryonic stem cell can differentiate into cardiomyocytes
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DOI：
10.1016/j.bbrc.2008.02.141
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发表时间：
2008-05-09
影响因子：
3.1
通讯作者：
Fukuda, Keiichi
中科院分区：
文献类型：
--
作者：
Chen, Hao;Hattori, Fumiyuki;Fukuda, Keiichi
普通绒猴作为灵长类动物的研究模型最近引起了人们的广泛关注，并且由于它们的小身体易于处理和高效繁殖而比恒河猴和食蟹猴更受欢迎。我们最近报道了普通绒猴胚胎干细胞（CMESC）系的建立，可以分化为三个胚层。在这里，我们报告，我们的CMESC也可以分化为心肌细胞，并调查他们的特点。诱导后表达FOG-2，然后是GATA 4和Tbx 20，然后是Nkx2.5和Tbx 5。在第12-15天可以检测到自发搏动。免疫荧光染色和超微结构分析表明，它们具有典型的功能性心肌细胞的特征。它们表现出窦房结样动作电位，异丙肾上腺素刺激可增加搏动频率。BrAU掺入试验显示，CMESC衍生的心肌细胞保持了高达24周的高增殖潜力。我们相信CMESC。衍生的心肌细胞将推进心血管再生医学的临床前研究。(c)2008年爱思唯尔公司All rights reserved.
Common marmoset monkeys have recently attracted much attention as a primate research model and, are preferred to rhesus and cynomolgus monkeys due to their small bodies easy handling and efficient, breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction FOG-2 was expressed followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrAU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC. derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine. (c) 2008 Elsevier Inc. All rights reserved.