Cross-reactivity of rabbit antibodies to lipopolysaccharides of Escherichia coli J5 and other gram-negative bacteria.
Cross-reactivity of rabbit antibodies to lipopolysaccharides of Escherichia coli J5 and other gram-negative bacteria.
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DOI：
10.1093/infdis/152.5.954
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发表时间：
1985-11
期刊：
影响因子：
--
通讯作者：
G. Siber;S. Kania;H. Warren
中科院分区：
文献类型：
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作者：
G. Siber;S. Kania;H. Warren
粗糙革兰氏阴性突变体如大肠杆菌J5和沙门氏菌Re595的抗血清被认为可以中和脂多糖（LPS）的毒性作用。为了验证这种抗血清能够结合异源内毒素，我们检查了在兔中诱导的针对各种LPS的IgG和IgM类抗体。免疫粗糙突变体或脂质A诱导高IgG抗体反应的同源纯化的LPS和相对较低，但显着的异源LPS。IgM抗体的增加也主要是同源LPS。用光滑生物体免疫诱导很少或没有异源LPS抗体。可溶性LPS，外膜囊泡，和整个细菌产生强烈的同源抑制，但很少或没有异源抑制酶联免疫吸附测定。抗血清对粗糙突变体的交叉吸附表明，异源LPS诱导的IgG和IgM抗体被异源LPS吸附，而不是被用于免疫动物的核心LPS吸附。针对J5或Re595 LPS的兔抗体不能以任何实质性程度结合异源LPS。用全细菌疫苗免疫，特别是粗糙突变体和脂质A，确实增加了对各种抗原的抗体。抗血清对粗糙突变体的保护作用是由于多克隆抗体反应或诱导尚未鉴定的因子的可能性值得进一步研究。
Antiserum to rough gram-negative mutants such as Escherichia coli J5 and Salmonella minnesota Re595 is thought to neutralize the toxic effects of lipopolysaccharides (LPSs). To verify that such antisera are capable of binding heterologous endotoxins, we examined IgG and IgM class antibodies induced in rabbits to a variety of LPSs. Immunization with rough mutants or lipid A induced high IgG antibody responses to the homologous purified LPS and relatively low but significant responses to heterologous LPSs. Increases in IgM antibodies were also primarily to homologous LPS. Immunization with smooth organisms induced little or no antibody to heterologous LPSs. Soluble LPS, outer membrane vesicles, and whole bacteria produced strong homologous inhibition but little or no heterologous inhibition in enzyme-linked immunosorbent assays. Cross-adsorption of antisera to rough mutants suggested that the IgG and IgM antibodies induced to heterologous LPS were adsorbed by the heterologous LPS and not by the core LPS used to immunize the animals. Rabbit antibody directed to J5 or Re595 LPS fails to bind to any substantial degree to heterologous LPS. Immunization with whole bacterial vaccines, particularly the rough mutants and lipid A, does increase antibody to a wide variety of antigens. The possibilities that the protective effects of antisera to rough mutants are due to a polyclonal antibody response or to the induction of as yet unidentified factor(s) deserve further investigation.