Membrane RNAs in bacteria.
Membrane RNAs in bacteria.
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细菌中的膜 RNA。
DOI：
10.1111/j.1365-2958.2010.07438.x
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发表时间：
2011
影响因子：
3.6
通讯作者：
Laederach,Alain
中科院分区：
文献类型：
--
作者：
Sanders,Wes;Laederach,Alain
OLE (Ornate Large Extremophile) rna构成了一个功能未知的大而保守的非编码转录本家族。在本期的《分子微生物学》杂志上，Kirsten Block、Ron Breaker及其同事开始解读OLE RNA的一些功能奥秘。这类RNA的长度约为610个核苷酸，平均核苷酸同一性为65%。OLE RNA家族是通过细菌基因组的比较序列分析发现的。它在嗜盐芽孢杆菌和梭菌目极端微生物中被发现。到目前为止，OLE RNA仅限于来自环境和人类肠道的厌氧菌。大量保守性良好的OLE RNA序列使得利用序列共变分析来准确预测OLE RNA二级结构成为可能（Puerta-Fernandez等，2006；Tseng等，2009；Weinberg等，2007；Weinberg等，2009）。OLE rna是目前已知存在于细菌中的最复杂的非编码rna之一（Weinberg et al., 2009）。OLE RNA是一个大而复杂的转录单元的一部分（图1A），该转录单元包含10个其他基因，包括OAP （OLE相关蛋白）。OAP蛋白是由位于OLE RNA基因下游的一个基因编码的。为了开始了解OLE RNA的生物学作用，Block和同事在体外分析了该RNA与嗜盐芽孢杆菌OAP之间的相互作用。通过凝胶移位实验，他们发现OAP和OLE形成了一个核糖核蛋白颗粒（RNP）。通过系统地去除OLE RNA的结构域，他们能够绘制RNA中OAP的结合位点。这种分析产生了一个高度保守的序列基序。OLE RNA和OAP共转录并形成RNP的事实表明这种相互作用在功能上很重要。
OLE (Ornate Large Extremophile) RNAs constitute a family of large, well-conserved noncoding transcripts of unknown function. In this issue of Molecular Microbiology, Kirsten Block, Ron Breaker and co-workers begin to decipher some of the functional mysteries surrounding OLE RNA. This class of RNA is approximately 610 nucleotides in length, with an average nucleotide identity of 65%. The OLE RNA family was discovered using comparative sequence analysis of bacterial genomes. It was found in Bacillus halodurans and in extremophiles of the Clostridiae order. So far, OLE RNA is confined to anaerobic bacteria from the environment and the human gut. The large number of well-conserved OLE RNA sequences has made it possible to use sequence co-variation analysis to accurately predict OLE RNA secondary structures (Puerta-Fernandez et al., 2006; Tseng et al., 2009; Weinberg et al., 2007; Weinberg et al., 2009). OLE RNAs are among the most complex noncoding RNAs currently known to exist in bacteria (Weinberg et al., 2009).OLE RNA is part of a large and complex transcriptional unit (Figure 1A) that contains 10 other genes including OAP (OLE-Associated Protein). The OAP protein is encoded by a gene located just downstream of the OLE RNA gene. To begin to understand the biological role of OLE RNA, Block and co-workers analyzed the interaction between the RNA and OAP of B. halodurans in vitro. Using gel shift assays, they found that OAP and OLE form a Ribo-Nucleo-protein Particle (RNP). By systematically removing domains of the OLE RNA, they were able to map the binding site of OAP in the RNA. This analysis yielded a sequence motif that is highly conserved. The fact that OLE RNA and OAP are cotranscribed and form an RNP suggests that this interaction is functionally important.