Distribution of Helicobacter pylori and Periodontopathic Bacterial Species in the Oral Cavity
Distribution of Helicobacter pylori and Periodontopathic Bacterial Species in the Oral Cavity
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DOI：
10.3390/biomedicines8060161
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发表时间：
2020-06-01
期刊：
影响因子：
4.7
通讯作者：
Nakano, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kadota, Tamami;Hamada, Masakazu;Nakano, Kazuhiko
口腔可能是幽门螺杆菌的储存库。然而，H.幽门克隆化未知。本文分析了H.幽门螺杆菌在口腔和主要的牙周病细菌物种。巢式PCR检测H. pylori和这些细菌物种在39名受试者的唾液、牙菌斑和牙髓的样本中。H.在7个牙菌斑样本（17.9%）、2个唾液样本（5.1%）和1个牙髓样本（2.6%）中检测到幽门螺杆菌。牙菌斑标本采集的牙齿周围的牙周袋，在H。pylori阳性率高于H. pylori阴性者（p < 0.05）。此外，牙龈卟啉单胞菌，一种主要的牙周病病原体，在H。pylori阳性率高于H. pylori阴性牙菌斑标本（p < 0.05）。与牙龈卟啉单胞菌牙周致病性有关的菌毛编码基因（fimA）在牙龈卟啉单胞菌和牙龈卟啉单胞菌中的分布不同。pylori阳性和H.幽门阴性受试者。我们的结论是H.幽门螺杆菌可以沿着特定的牙周病细菌种类存在于口腔中，尽管其与这些细菌的相互作用尚不清楚。
The oral cavity may serve as a reservoir of Helicobacter pylori. However, the factors required for H. pyloricolonization are unknown. Here, we analyzed the relationship between the presence of H. pylori in the oral cavity and that of major periodontopathic bacterial species. Nested PCR was performed to detect H. pylori and these bacterial species in specimens of saliva, dental plaque, and dental pulp of 39 subjects. H. pylori was detected in seven dental plaque samples (17.9%), two saliva specimens (5.1%), and one dental pulp (2.6%) specimen. The periodontal pockets around the teeth, from which dental plaque specimens were collected, were significantly deeper in H. pylori-positive than H. pylori-negative subjects (p < 0.05). Furthermore,Porphyromonas gingivalis, a major periodontopathic pathogen, was detected at a significantly higher frequency in H. pylori-positive than in H. pylori-negative dental plaque specimens (p < 0.05). The distribution of genes encoding fimbriae (fimA), involved in the periodontal pathogenicity of P. gingivalis, differed between H. pylori-positive and H. pylori-negative subjects. We conclude that H. pylori can be present in the oral cavity along with specific periodontopathic bacterial species, although its interaction with these bacteria is not clear.