Integrated analysis of microRNA and mRNA expression profiles in HBx-expressing hepatic cells.
Integrated analysis of microRNA and mRNA expression profiles in HBx-expressing hepatic cells.
复制标题
表达 HBx 的肝细胞中 microRNA 和 mRNA 表达谱的综合分析
DOI：
10.3748/wjg.v23.i10.1787
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发表时间：
2017-03-14
影响因子：
4.3
通讯作者：
Fan XG
中科院分区：
文献类型：
--
作者：
Chen RC;Wang J;Kuang XY;Peng F;Fu YM;Huang Y;Li N;Fan XG
目的：研究B X型肝炎病毒（HBx）表达肝细胞中miRNA-mRNA的调控网络。建立了稳定表达HBx的人肝细胞系L02。RNA测序分析L02/HBx和L02/pcDNA肝细胞的mRNA和miRNA表达谱。通过京都基因百科全书和基因组途径富集分析来研究候选生物标志物的功能，并通过网络分析来研究miRNA和mRNA之间的关系。与L02/pcDNA细胞相比，L02/HBx细胞中有742个基因表达上调，501个基因表达下调。基因本体论分析表明，差异表达基因与不同的生物学过程有关。同时，还在L02/HBx细胞中测定了22种差异miRNA。此外，miRNA和mRNA表达谱的综合分析确定了与HBx致癌作用相关的核心miRNA-mRNA调控网络。总的来说，基于miRNA-mRNA网络的分析可能有助于阐明HBx在肝细胞恶性转化中的潜在作用，并阐明肝细胞癌的潜在分子机制和新的治疗靶点。
To identify the miRNA-mRNA regulatory network in hepatitis B virus X (HBx)-expressing hepatic cells. A stable HBx-expressing human liver cell line L02 was established. The mRNA and miRNA expression profiles of L02/HBx and L02/pcDNA liver cells were identified by RNA-sequencing analysis. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed to investigate the function of candidate biomarkers, and the relationship between miRNA and mRNA was studied by network analysis. Compared with L02/pcDNA cells, 742 unregulated genes and 501 downregulated genes were determined as differentially expressed in L02/HBx cells. Gene ontology analysis suggested that the differentially expressed genes were relevant to different biological processes. Concurrently, 22 differential miRNAs were also determined in L02/HBx cells. Furthermore, integrated analysis of miRNA and mRNA expression profiles identified a core miRNA-mRNA regulatory network that is correlated with the carcinogenic role of HBx. Collectively, the miRNA-mRNA network-based analysis could be useful to elucidate the potential role of HBx in liver cell malignant transformation and shed light on the underlying molecular mechanism and novel therapy targets for hepatocellular carcinoma.