Characterization of inulinase promoter from Kluyveromyces marxianus for intensive protein expression in industrial biotechnology
Characterization of inulinase promoter from Kluyveromyces marxianus for intensive protein expression in industrial biotechnology
复制标题
DOI：
10.1093/femsyr/fox062
复制
发表时间：
2017-09-01
影响因子：
3.2
通讯作者：
Jiang, Yu
中科院分区：
文献类型：
--
作者：
Gao, Jiaoqi;Yuan, Wenjie;Jiang, Yu
马氏克鲁维菌的菊粉酶（KmINU1）在工业生物技术中有着广泛的应用，其表达可能在转录水平上受启动子调控。因此，本研究分析了融合到报告基因EGFP的KmINU1启动子，以确定其在Saccharomyces cerevisiae S288C （ATCC 204508）中的基本调控机制。据报道，菊粉对KmINU1启动子的诱导作用和高葡萄糖浓度对KmINU1启动子的抑制作用，其中长度为353 bp的启动子强度最大，对高葡萄糖浓度的反应最弱。KmINU1启动子对碳源的响应与Mig1结合位点（-496 ~ -485 bp）有关。因此，我们提出明确菊粉酶启动子的调控机制，为其在蛋白质生产中的应用提供有力的理论参考。
Inulinase from Kluyveromyces marxianus (KmINU1) has wide application in industrial biotechnology, and it is supposed that its expression is regulated at the transcriptional level via the promoter. Therefore, in this study, the KmINU1 promoter, fused to the reporter EGFP gene, was analyzed to determine its fundamental regulatory mechanisms in Saccharomyces cerevisiae S288C (ATCC 204508). Induction by inulin and repression by high glucose concentrations of KmINU1 promoters are reported, and the promoter with a length of 353 bp was shown to have the highest strength with the weakest responses to high glucose concentration. Responses of the KmINU1 promoter to carbon source were shown to be related to the Mig1 binding site extending from -496 to -485 bp. Hence, we propose clear regulation mechanisms of the inulinase promoter, which may provide a powerful theoretical reference for its application in protein production.