Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb
Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb
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DOI：
10.1002/gene.20146
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发表时间：
2005-09-01
期刊：
影响因子：
1.5
通讯作者：
Yokoi, M
中科院分区：
文献类型：
--
作者：
Nagai, Y;Sano, H;Yokoi, M
嗅觉信息通过嗅球中的二尖瓣和簇状 (M/T) 细胞从外周传递到嗅皮质。在 M/T 细胞中特异性表达 Cre 重组酶的小鼠对于 M/T 细胞的遗传标记和操纵其特性至关重要。原钙粘蛋白 21 (Pcdh21) 的表达高度局限于 M/T 细胞。在这里，我们报道了一个转基因小鼠品系，Pcdh21-Cre，其中类似于10-kb小鼠Pcdh21启动子驱动Cre重组酶的表达。在 Pcdh21-Cre 小鼠中，Cre 重组酶活性主要在 M/T 细胞中检测到，通过 Pcdh21-Cre 与报告基因 Rosa26-IoxP-stop-IoxP-CFP 菌株杂交后代的抗 CFP 免疫染色进行可视化。这些结果表明，类似10-kb Pcdh21启动子可以驱动M/T细胞中的转录，并且Pcdh21-Cre小鼠可用于切除M/T细胞中的floxed DNA片段，这为揭示中枢嗅觉回路的结构和功能提供了有价值的工具。
Olfactory information is conveyed from the periphery to the olfactory cortices through mitral and tufted (M/T) cells in the olfactory bulb. A mouse with a specific expression of Cre recombinase in M/T cells is essential for genetic marking of M/T cells and manipulating their properties. Protocadherin 21 (Pcdh21) expression is highly restricted to M/T cells. Here we report a transgenic mouse line, Pcdh21-Cre, in which similar to 10-kb mouse Pcdh21 promoter drives the expression of Cre recombinase. In Pcdh21-Cre mice, Cre recombinase activity is predominantly detected in M/T cells, visualized with the anti-CFP immunostaining in offspring of a cross between Pcdh21-Cre and the reporter Rosa26-IoxP-stop-IoxP-CFP strain. These results demonstrate that the similar to 10-kb Pcdh21 promoter can drive transcription in M/T cells and Pcdh21-Cre mice can be used to excise floxed DNA fragments in M/T cells, which provides a valuable tool to reveal the structure and function of the central olfactory circuits.