The population genetics of a biological control introduction:: mitochondrial DNA and microsatellie variation in native and introduced populations ofAphidus ervi, a parisitoid wasp
The population genetics of a biological control introduction:: mitochondrial DNA and microsatellie variation in native and introduced populations ofAphidus ervi, a parisitoid wasp
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DOI：
10.1046/j.1365-294x.2003.02084.x
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发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Harrison, RG
中科院分区：
文献类型：
--
作者：
Hufbauer, RA;Bogdanowicz, SM;Harrison, RG
引进生物防治剂可能会造成种群规模的瓶颈，尽管人们努力避免它们。我们研究了种群遗传学的蚜虫ervi（Hyplutera：茧蜂科），一种寄生蜂，介绍了北美从西欧在1959年，以控制豌豆蚜虫。探讨了A.我们对27个来自本地范围的个体和51个来自引入范围的个体的线粒体DNA（mtDNA）进行了1249 bp的测序。大多数来自西欧、中东和北美的个体都有两个共同的单倍型之一，这与已知的引进历史一致。然而，一些A.来自太平洋西北部的ervi具有与日本发现的单倍型最相似的单倍型，这增加了第二次意外引入的可能性。为了研究种群结构，并评估是否发生瓶颈后，引入北美，我们分析了5个微卫星位点的变化，62个个体从2个本地种群和230个个体从6个引进的种群。引入的样品比本地样品具有更少的稀有等位基因（F-1，F-34 = 13.5，P = 0.0008），但杂合性没有显著差异。这些结果表明，一个温和的瓶颈发生，尽管超过1000人的介绍。使用分层贝叶斯方法，估计初始人群规模为245人。AMOVA显示欧洲和北美样本之间存在显着的遗传分化，贝叶斯分配方法将个体聚类为四组，其中一组中的大多数欧洲个体和其他三组中的大多数北美个体。这些结果突出表明，遗传变化与快速增长的自然种群中的创始人事件有关，即使创始种群规模相对较大。
Introductions of biological control agents may cause bottlenecks in population size despite efforts to avoid them. We examined the population genetics of Aphidius ervi (Hymenoptera: Braconidae), a parasitoid that was introduced to North America from Western Europe in 1959 to control pea aphids. To explore the phylogeographical relationships of A. ervi we sequenced 1249 bp of mitochondrial DNA (mtDNA) from 27 individuals from the native range and 51 individuals from the introduced range. Most individuals from Western Europe, the Middle East and North America shared one of two common haplotypes, consistent with the known history of the introduction. However, some A. ervi from the Pacific Northwest have a haplotype that is most similar to haplotypes found in Japan, raising the possibility of a second accidental introduction. To examine population structure and assess whether a bottleneck occurred upon introduction to North America, we assayed variation at 5 microsatellite loci in 62 individuals from 2 native populations and 230 individuals from 6 introduced populations. Introduced samples had fewer rare alleles than native samples (F-1,F-34 = 13.5, P = 0.0008), but heterozygosity did not differ significantly. These results suggest that a mild bottleneck occurred in spite of the introduction of over 1000 individuals. Using a hierarchical Bayesian approach, the founding population size was estimated to be 245 individuals. AMOVA showed significant genetic differentiation between the European and North American samples, and a Bayesian assignment approach clustered individuals into four groups, with most European individuals in one group and most North American individuals in the other three. These results highlight that genetic changes are associated with founder events in rapidly growing natural populations, even when the founding population size is relatively large.