Differential expression profile of cold (TRPA1) and cool (TRPM8) receptors in human urogenital organs
Differential expression profile of cold (TRPA1) and cool (TRPM8) receptors in human urogenital organs
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DOI：
10.1016/j.urology.2007.11.127
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发表时间：
2008-08-01
期刊：
影响因子：
2.1
通讯作者：
Takeda, Masayuki
中科院分区：
文献类型：
--
作者：
Du, Shuqi;Araki, Isao;Takeda, Masayuki
目的 检查人类泌尿生殖器官（膀胱和前列腺）中瞬时受体电位 (TRP) 通道 A1 和 TRPM8 的表达，以及这些表达如何与膀胱出口梗阻 (BOO) 或良性前列腺增生 (BPH) 相关。除了TRPM8这种冷受体之外，TRPA1最近也被认为是一种冷受体。 TRPA1 也是机械传感器和/或伤害感受器的候选者。方法 膀胱粘膜和肌层取自 9 名对照者和 9 名 BOO 患者。前列腺标本取自 5 名对照者和 6 名 BPH 患者。通过实时里维尔转录酶-聚合酶链式反应对 TRPA1 和 TRPM8 信使 RNA (mRNA) 的表达进行定量。采用免疫组化方法探讨TRPA1蛋白的定位。结果TRPA1 mRNA在膀胱黏膜、膀胱肌层、前列腺中的表达量比例为639:1:16。 BOO组膀胱粘膜TRPA1 mRNA显着上调至对照的2.32倍。 TRPA1蛋白定位于膀胱和前列腺的上皮细胞中。 TRPM8 mRNA在前列腺中的表达量远高于膀胱粘膜中的表达量(3024:1)，但在膀胱肌层中未发现TRPM8 mRNA的表达量。 BPH或BOO对TRPM8的表达没有显着影响。结论TRPA1和TRPM8在人膀胱和前列腺中存在差异表达。膀胱上皮中的TRPA1可能参与膀胱感觉转导以及BOO诱发膀胱过度活动症的过程。
OBJECTIVES To examine the expressions of transient receptor potential (TRP) channel A1 and TRPM8 in the human urogenital organs (urinary bladder and prostate) and how these expressions change in association with bladder outlet obstruction (BOO) or benign prostatic hyperplasia (BPH). In addition to TRPM8, a cool receptor, TRPA1 is recently recognized as a cold receptor. TRPA1 is also a candidate for mechanosensor and/or nociceptor.METHODS Urinary bladder mucosa and muscular layer were taken from 9 controls and 9 patients with BOO. Prostatic specimens were obtained from 5 controls and 6 patients with BPH. Expressions of TRPA1 and TRPM8 messenger RNAs (mRNAs) were quantified by real-time revere transcriptase-polymerase chain reaction. Localization of TRPA1 protein was explored with immunohistochemistry.RESULTS The expression levels of TRPA1 mRNA in the bladder mucosa, bladder muscular layer, and prostate were in the ratio of 639:1:16. TRPA1 mRNA in the bladder mucosa with BOO was significantly upregulated to 2.32 times of control. TRPA1 protein was localized in the epithelial cells of both urinary bladder and prostate gland. The expression of TRPM8 mRNA in the prostate was much higher than that in the bladder mucosa (3024:1), but was not found in the bladder muscle layer. BPH or BOO did not significantly affect the expression of TRPM8.CONCLUSIONS TRPA1 and TRPM8 were differentially expressed in the human urinary bladder and prostate. TRPA1 in the bladder epithelium might be involved in the bladder sensory transduction and the induction process of overactive bladder by BOO.