Oral Microbiota in Severe Early Childhood Caries in Thai Children and Their Families: A Pilot Study
Oral Microbiota in Severe Early Childhood Caries in Thai Children and Their Families: A Pilot Study
复制标题
DOI：
10.3389/fmicb.2018.02420
复制
发表时间：
2018-10-15
影响因子：
5.2
通讯作者：
McBain, Andrew J.
中科院分区：
文献类型：
--
作者：
Ledder, Ruth G.;Kampoo, Kanokporn;McBain, Andrew J.
泰国儿童严重龋齿（S-ECC）的患病率相对较高。S-ECC对儿童及其照顾者的生活质量产生不利影响，并造成相当大的经济负担。我们评估了泰国队列中未受刺激的唾液、牙菌斑和降解牙本质的细菌学组成，包括S-ECC儿童和无龋洞儿童;他们的兄弟姐妹和他们的主要照顾者。在牙科检查中收集样本，并对患者的牙菌斑积累和龋齿、缺失和填充牙（dmft）指数进行评分。使用细菌学差异计数和基于凝胶的真细菌DNA谱分析样品。菌斑乳酸菌丰度与S-ECC显著相关。虽然S-ECC儿童的乳酸菌计数显著高于其兄弟姐妹和主要照顾者（5个家庭），但无空洞儿童的趋势相反。S-ECC患儿的革兰氏阴性厌氧菌计数显著低于口腔健康儿童。S-ECC与菌斑指数评分、dmft和乳酸菌丰度高度相关。DNA图谱显示家族间有显著的同源性，但非空洞组和S-ECC组间无同源性。总之，唾液和菌斑乳酸菌计数与泰国受试者的S-ECC显著相关。儿童的乳酸菌计数与他们的兄弟姐妹和主要照顾者的乳酸菌计数无关。个人可以根据家庭而不是龋齿状况进行显着区分。
Thailand has a comparatively high prevalence of severe early childhood caries (S-ECC). S-ECC adversely affects the quality of life for children and their caregivers and represents a considerable economic burden. We have assessed the bacteriological composition of unstimulated saliva, dental plaque, and degraded dentine in a Thai cohort, including children with S-ECC and children without cavities; their siblings, and their primary caregivers. Samples were collected during a dental examination and patients were scored for plaque accumulation and their decayed, missing, and filled teeth (dmft) index. Samples were analyzed using differential bacteriological counting and gel-based eubacterial DNA profiling. Plaque Lactobacillus abundance correlated significantly with S-ECC. Whilst Lactobacillus counts were significantly higher in children with S-ECC than in their siblings and primary caregivers (five families), the opposite trend was apparent for cavity-free children. Counts of Gram-negative anaerobes were significantly lower in children with S-ECC than orally healthy children. S-ECC correlated significantly with plaque index scores, dmft, and with Lactobacillus abundance in a highly predictive manner. DNA profiles showed significant homology between families but not within non-cavity and S-ECC groups. In conclusion, salivary and plaque Lactobacillus counts were significantly associated with S-ECC in the Thai subjects. Lactobacillus counts in the children were not correlated with those of their siblings and primary caregivers. Individuals could be significantly differentiated based on family but not on caries status.