Role of bta-miR-204 in the regulation of adipocyte proliferation, differentiation, and apoptosis
Role of bta-miR-204 in the regulation of adipocyte proliferation, differentiation, and apoptosis
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bta-miR-204 在脂肪细胞增殖、分化和凋亡调节中的作用
DOI：
10.1002/jcp.27928
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发表时间：
2019-07-01
影响因子：
5.6
通讯作者：
Lan, Xianyong
中科院分区：
文献类型：
--
作者：
Jin, Yunyun;Wang, Jian;Lan, Xianyong
脂肪细胞的生长和发育是复杂和精确协调的过程。一些microRNAs已被确定为脂肪细胞生长和发育的关键调节因子。最近，BTA-miR-204被发现参与脂肪形成，但BTA-miR-204调控脂肪细胞增殖、分化和凋亡的分子机制尚不完全清楚或阐明。本研究采用实时定量聚合酶链式反应(qRT-PCR)、细胞计数试剂盒-8、EDU、流式细胞仪、油红O染色和免疫印迹等方法检测BTA-miR-204在脂肪细胞生长发育中的作用。过表达BTA-miR-204对3T3-L1细胞的增殖无明显影响。BTA-miR-204强制表达促进3T3-L1细胞分化。同时，BTA-miR-204在信使RNA和蛋白水平上上调Bax的表达，下调Bcl-2的表达，提示BTA-miR-204具有促进3T3-L1细胞凋亡的作用。通过生物信息学分析、双荧光素酶报告系统和qRT-PCR等方法，确定TGFBR2和ELOVL6为BTA-miR-204的直接靶基因。因此，我们的研究为BTA-miR-204在脂肪细胞生长发育调控中的作用提供了新的视角，可能为治疗肥胖症提供新的选择。
Adipocyte growth and development are complex and precisely orchestrated processes. Several microRNAs have been identified as critical regulators of the adipocyte growth and development. Recently, bta-miR-204 was found to be involved in adipogenesis; however, the underlying molecular mechanism involved in bta-miR-204-mediated regulation of proliferation, differentiation, and apoptosis of adipocytes is not fully understood or elucidated. In this study, quantitative real-time polymerase chain reaction (qRT-PCR), Cell Counting Kit-8, EdU, flow cytometer, Oil Red O staining, and the western blot assays were used to assess the role of bta-miR-204 in adipocyte growth and development. Overexpression of bta-miR-204 had no significant effect on 3T3-L1 cell proliferation. The forced expression of bta-miR-204 promoted 3T3-L1 cell differentiation. Meanwhile, overexpression of bta-miR-204 upregulated the expression of Bax and downregulated the expression of Bcl-2 both at messenger RNA and protein levels, which suggested that bta-miR-204 can promote 3T3-L1 cell apoptosis. Using bioinformatic analysis, dual-luciferase reporter system and qRT-PCR, TGFBR2, and ELOVL6 were identified as the direct target genes of bta-miR-204. Therefore, our study provides a novel insight into the role of bta-miR-204 in the regulation of adipocyte growth and development, which may provide a novel therapeutic alternative against obesity.