ENERGY-EXPENDITURE BY DOUBLY LABELED WATER - VALIDATION IN LEAN AND OBESE SUBJECTS
ENERGY-EXPENDITURE BY DOUBLY LABELED WATER - VALIDATION IN LEAN AND OBESE SUBJECTS
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DOI：
10.1152/ajpendo.1991.261.3.e402
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发表时间：
1991-09-01
影响因子：
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通讯作者：
BOGARDUS, C
中科院分区：
文献类型：
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作者：
RAVUSSIN, E;HARPER, IT;BOGARDUS, C
双标记水((H2O)-H-2-O-18)方法用于评估自由生活条件下的能量消耗，已成功地与瘦健康志愿者在久坐和持续大强度运动中的气体交换测量进行了验证。然而，目前还没有关于肥胖受试者的数据。因此，我们在12名男性受试者中比较了(H2O)-H-2-O-18方法和间接量热法(呼吸室)，受试者的体重和成分范围广泛(61-190公斤，7-41%脂肪)。使用多点斜率/截距方法计算基线尿样、7h、11.5h和每日剂量后尿样中O-18和H-2浓集的同位素池大小和清除率。对结果进行了同位素分馏校正。呼吸室的平均7天能量消耗从1851大卡/天到4105大卡/天不等。双标记水法倾向于低估能量消耗(-2.5+/-5.8%，不等于0，范围-14至+4%)，较大的低估发生在较重和较胖的受试者(r分别为-0.82和-0.68，P<；0.02)。体重较重的受试者的低估可能与脂肪合成过程中较大的氢封存有关。总而言之，双标记水法是测量自由生活条件下能量消耗的一种合适而准确的方法，但在较胖的受试者中可能会略有低估。
The doubly labeled water ((H2O)-H-2-O-18) method to assess energy expenditure in free-living conditions has been successfully validated against gas exchange measurements in lean healthy volunteers in both sedentary conditions and during sustained heavy exercise. However, no data are available on obese subjects. We therefore compared the (H2O)-H-2-O-18 method with indirect calorimetry (respiratory chamber) in 12 male subjects covering a wide range of body weight and composition (61-190 kg, 7-41% fat). Isotope pool sizes and elimination rates were calculated from O-18 and H-2 enrichments in baseline urine samples and in 7-h, 11.5-h, and daily postdose urine samples using the multipoint slope/intercept method. Results were corrected for isotopic fractionation. Mean 7-day energy expenditure in the respiratory chamber varied from 1,851 to 4,105 kcal/day. The doubly labeled water method tended to underestimate energy expenditure (-2.5 +/- 5.8%, not-equal 0, range -14 to +4%), with the larger underestimate observed in heavier and fatter subjects (r = -0.82 and -0.68, P < 0.02, respectively). The underestimation in heavier subjects might be related to larger sequestration of deuterium during fat synthesis. In conclusion, the doubly labeled water method is a suitable and accurate method to measure energy expenditure in free-living conditions but might provide a slightly underestimated figure in fatter subjects.