SPECIFIC MODULATION OF DOPAMINE EXPRESSION IN NEURONAL HYBRID-CELLS BY PRIMARY-CELLS FROM DIFFERENT BRAIN-REGIONS
SPECIFIC MODULATION OF DOPAMINE EXPRESSION IN NEURONAL HYBRID-CELLS BY PRIMARY-CELLS FROM DIFFERENT BRAIN-REGIONS
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DOI：
10.1073/pnas.89.19.8943
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发表时间：
1992-10-01
影响因子：
11.1
通讯作者：
HELLER, A
中科院分区：
文献类型：
--
作者：
CHOI, HK;WON, L;HELLER, A
MN 9D是一种含有多巴胺的永生化神经元杂交细胞系。当MN 9D细胞与视顶盖的原代胚胎细胞共聚集时，视顶盖是一个不接受多巴胺能神经支配的脑区，其多巴胺含量、酪氨酸羟化酶免疫反应性和酪氨酸羟化酶mRNA明显减少。与胚胎丘脑（另一个缺乏多巴胺能神经支配的大脑区域）细胞的共聚集也产生了类似的多巴胺含量减少。MN 9D细胞与纹状体或皮质的多巴胺感受细胞的共聚集对MN 9D细胞的多巴胺含量没有明显的刺激作用。视顶盖细胞产生的MN 9D多巴胺含量的减少并没有被纹状体细胞逆转。因此，MN 9D杂合细胞能够响应来自不是多巴胺能神经元靶的脑区域的细胞的抑制因子。产生儿茶酚胺的PC 12细胞没有以类似的方式响应，这表明MN 9D细胞的响应是其中脑起源的功能。鉴于MN 9D细胞对不同脑细胞群的选择性反应，该杂交细胞系应有助于研究中枢神经系统中可能参与神经递质表型表达和特定神经元连接建立的细胞-细胞相互作用。
MN9D is an immortalized dopamine-containing neuronal hybrid cell line. When MN9D cells were coaggregated with primary embryonic cells of optic tectum, a brain region that does not receive a dopaminergic innervation, there was a marked reduction in their dopamine content, tyrosine hydroxylase immunoreactivity, and tyrosine hydroxylase mRNA. Similar reductions in dopamine content were produced by coaggregation with cells from embryonic thalamus, another brain region devoid of dopaminergic innervation. Coaggregation of MN9D cells with dopaminoceptive cells from the corpus striatum or the cortex did not have a demonstrable stimulatory effect on the dopamine content of MN9D cells. The decrease in MN9D dopamine content produced by optic tectum cells was not reversed by addition of corpus striatum cells. Thus, the MN9D hybrid cells are able to respond to an inhibitory factor(s) from cells derived from brain areas that are not targets for dopaminergic neurons. Catecholamine-producing PC12 cells did not respond in a similar manner, suggesting that the response of MN9D cells is a function of their mesencephalic origin. Given the selective response of MN9D cells to different brain cell populations, this hybrid cell line should facilitate investigations of cell-cell interactions in the central nervous system that may be involved in the expression of neurotransmitter phenotype and establishment of specific neuronal connections.