β‐Glucocerebrosidase Activity in the Stratum Corneum of House Sparrows following Acclimation to High and Low Humidity
β‐Glucocerebrosidase Activity in the Stratum Corneum of House Sparrows following Acclimation to High and Low Humidity
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适应高湿度和低湿度后麻雀角质层中的β-葡萄糖脑苷脂酶活性
DOI：
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发表时间：
2007
影响因子：
1.6
通讯作者：
Joseph B. Williams
中科院分区：
文献类型：
--
作者：
R. M. Cox;A. Muñoz;M. Jurkowitz;Joseph B. Williams
皮肤是陆栖鸟类失水的重要途径，因此需要保持水分的环境条件应该有利于减少皮肤失水(CWL)的生理机制。皮肤对CWL的抵抗力是由位于角质层(SC)的脂质分子屏障所致，角质层是表皮的外层。在哺乳动物中，SC屏障的功能依赖于β-葡萄糖脑苷酶(β-GlcCer‘ase)将脑苷脂转化为神经酰胺。禽类SC中同时含有脑苷脂和神经酰胺，提示CWL中观察到的可塑性可能是通过β-GlcCer‘酶活性和所产生的SC脂组成的变化来调节的。我们通过将家雀(Passer Home Ticus)驯化到干燥(6.5g H2O mβ3绝对湿度)或潮湿(31g H2O m−3)条件下，在30℃下分别培养5和21d，然后测定SC匀浆中的−-GlcCer‘酶活性，从而检验了环境湿度变化会改变β-GlcCer’ase活性的假设。我们的结果首次提供了非哺乳动物脊椎动物β-GlcCer‘酶活性的特征。与未驯化的对照相比，干燥驯化和潮湿驯化的麻雀在驯化21d后β-GlcCer‘酶活性均显著升高。在个体间，我们观察到β-GlcCer‘酶活性与CWL和SC神经酰胺含量呈负相关。尽管干驯化和湿驯化麻雀的β-GlcCer‘酶活性没有差异，但这些结果与我们的发现一致，即两种湿度处理都导致了CWL的减少和SC脂肪组成的相似变化。我们的结果证明了慢性水肿病的生理可塑性，并初步支持了β-GlcCer酶在调节这一反应中的作用。
Skin is an important avenue of water loss in terrestrial birds, so environmental conditions that necessitate water conservation should favor physiological mechanisms that reduce cutaneous water loss (CWL). Skin resistance to CWL is conferred by a barrier of lipid molecules located in the stratum corneum (SC), the outer layer of the epidermis. In mammals, SC barrier function depends on the conversion of cerebrosides to ceramides by the enzyme β‐glucocerebrosidase (β‐GlcCer’ase). Avian SC contains both cerebrosides and ceramides, suggesting that observed plasticity in CWL may be mediated by changes in β‐GlcCer’ase activity and resultant SC lipid composition. We tested the hypothesis that changes in ambient humidity would alter β‐GlcCer’ase activity by acclimating house sparrows (Passer domesticus) to either dry (6.5 g H2O m−3 absolute humidity) or humid (31 g H2O m−3) conditions for 5 and 21 d at 30°C and then measuring β‐GlcCer’ase activity from SC homogenates. Our results provide the first characterization of β‐GlcCer’ase activity in any nonmammalian vertebrate. Relative to nonacclimated controls, both dry‐ and humid‐acclimated sparrows had significantly elevated β‐GlcCer’ase activity at 21 d postacclimation. Across individuals, we observed negative correlations between β‐GlcCer’ase activity and both CWL and SC ceramide content. Although dry‐ and humid‐acclimated sparrows did not differ in β‐GlcCer’ase activity, these results are consistent with our findings that both humidity treatments caused a reduction in CWL and similar changes in SC lipid composition. Our results demonstrate physiological plasticity in CWL and provide tentative support for a role of β‐GlcCer’ase in mediating this response.
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哺乳动物葡萄糖脑苷脂酶：对戈谢病的影响。
DOI：
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发表时间：
1988
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Glew,RH;Basu,A;LaMarco,KL;Prence,EM
通讯作者：
Prence,EM
渗透屏障的要求调节表皮β-葡萄糖脑苷脂酶。
影响因子：
6.5
作者：
Holleran,WM;Takagi,Y;Menon,GK;Jackson,SM;Lee,JM;Feingold,KR;Elias,PM
通讯作者：
Elias,PM
影响因子：
6.5
作者：
Y. Takagi;E. Kriehuber;G. Imokawa;P. Elias;W. Holleran
通讯作者：
Y. Takagi;E. Kriehuber;G. Imokawa;P. Elias;W. Holleran
影响因子：
15.9
作者：
HOLLERAN, WM;GINNS, EI;SIDRANSKY, E
通讯作者：
SIDRANSKY, E
鼠表皮中的β-葡萄糖脑苷脂酶活性：与分化相关的表征和定位。
影响因子：
6.5
作者：
Holleran,WM;Takagi,Y;Imokawa,G;Jackson,S;Lee,JM;Elias,PM
通讯作者：
Elias,PM