Temperature Sensing Using Fluorescent Nanothermometers
Temperature Sensing Using Fluorescent Nanothermometers
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DOI：
10.1021/nn100244a
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发表时间：
2010-06-01
期刊：
影响因子：
17.1
通讯作者：
Capobianco, John A.
中科院分区：
文献类型：
--
作者：
Vetrone, Fiorenzo;Naccache, Rafik;Capobianco, John A.
获取单个活细胞的温度并不是一项简单的任务。在本文中，我们设计了一种新型纳米温度计，能够准确测定溶液以及 HeLa 癌细胞等生物系统的温度。该纳米温度计基于NaYF4:Er3+,Yb3+纳米颗粒的温度敏感荧光，其中Er3+掺杂离子（H-2(11/2) -> I-4(15/2)和S-4(3/2) -> I-4(15/2)）的绿色荧光带的强度比随温度变化。纳米温度计首先用于通过泵探针实验获得加热 NaYF4:Er3+,Yb3+ 纳米粒子在水中的胶体溶液时产生的热分布。将纳米颗粒与 HeLa 宫颈癌细胞一起孵育并随后被摄取后，荧光纳米温度计测量了活细胞的内部温度，从 25 摄氏度到 45 摄氏度热诱导死亡。
Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.