Hydrogels in Electrophoresis: Applications and Advances
Hydrogels in Electrophoresis: Applications and Advances
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DOI：
10.2116/analsci.20r004
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发表时间：
2020-10
影响因子：
1.6
通讯作者：
Chenchen Liu;T. Kubo;K. Otsuka
中科院分区：
文献类型：
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作者：
Chenchen Liu;T. Kubo;K. Otsuka
水凝胶是吸收在大量水溶液中的聚合物网络的固体形式。在电泳中，水凝胶发挥多种作用，包括作为支持介质、筛分基质、亲和支架和分子印迹聚合物的组合物。近年来，水凝胶的研究以前所未有的速度推进，水凝胶在分离科学中的应用带来了新的机遇和可能的突破。充分了解材料的作用和效果对于水凝胶应用至关重要。本综述总结了各种电泳分离模式中描述的水凝胶，包括等电聚焦凝胶电泳 (IEFGE)、等速电泳 (ITP)、凝胶电泳和亲和凝胶电泳 (AGE)。由于微芯片电泳（ME）是电泳的未来趋势之一，因此还介绍了与ME中的水凝胶相关的发人深省的研究。重点介绍了提高分离性能、促进实验操作过程、允许快速分析以及促进与微流体装置集成的新型水凝胶和方法。
A hydrogel is a solid form of polymer network absorbed in a substantial amount of aqueous solution. In electrophoresis, hydrogels play versatile roles including as support media, sieving matrixes, affinity scaffolds, and compositions of molecularly imprinting polymers. Recently, the study of hydrogels has been advancing with unprecedented speed, and the application of hydrogels in separation science has brought new opportunities and possible breakthroughs. A good understanding about the roles and effects of the material is essential for hydrogel applications. This review summarizes the hydrogels that has been described in various modes of electrophoretic separations, including isoelectric focusing gel electrophoresis (IEFGE), isotachophoresis (ITP), gel electrophoresis and affinity gel electrophoresis (AGE). As microchip electrophoresis (ME) is one of the future trends in electrophoresis, thought provoking studies related to hydrogels in ME are also introduced. Novel hydrogels and methods that improve separation performance, facilitate the experimental operation process, allow for rapid analysis, and promote the integration to microfluidic devices are highlighted.