NUCLEAR-LOCALIZATION OF AGROBACTERIUM VIRE2 PROTEIN IN PLANT-CELLS
NUCLEAR-LOCALIZATION OF AGROBACTERIUM VIRE2 PROTEIN IN PLANT-CELLS
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DOI：
10.1126/science.1615325
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发表时间：
1992-06-26
期刊：
影响因子：
56.9
通讯作者：
ZAMBRYSKI, P
中科院分区：
文献类型：
--
作者：
CITOVSKY, V;ZUPAN, J;ZAMBRYSKI, P
农杆菌单链DNA （ssDNA）中间t链可能作为一个复合体转移到植物细胞核中，在其5'端有一个VirD2分子，在其长度上有多个VirE2分子。VirD2含有一个核定位信号（NLS）；然而，由于t链主要被VirE2分子包裹，因此VirE2也可能有助于核摄取。事实上，与报告蛋白融合的VirE2可以定位到植物细胞核，这一过程是由两个与爪蟾核纤溶蛋白双侧NLS同源的氨基酸序列介导的。此外，将一种无毒的virE2突变体接种到表达virE2的转基因植物上后，其致瘤性得以恢复，从而支持了virE2在植物中的功能。
The Agrobacterium single-stranded DNA (ssDNA) intermediate T-strand is likely transferred to the plant cell nucleus as a complex with a single VirD2 molecule at its 5' end and multiple VirE2 molecules along its length. VirD2 contains a nuclear localization signal (NLS); however, because the T-strand is principally coated with VirE2 molecules, VirE2 also might assist in nuclear uptake. Indeed, VirE2 fused to a reporter protein localizes to plant cell nuclei, a process mediated by two amino acid sequences with homology to the bipartite NLS of Xenopus nucleoplasmin. Moreover, tumorigenicity of an avirulent virE2 mutant is restored when inoculated on transgenic plants expressing VirE2, supporting in planta function of VirE2.