Periderm cells covering palatal shelves have tight junctions and their desquamation reduces the polarity of palatal shelf epithelial cells in palatogenesis
Periderm cells covering palatal shelves have tight junctions and their desquamation reduces the polarity of palatal shelf epithelial cells in palatogenesis
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DOI：
10.1111/j.1365-2443.2012.01601.x
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发表时间：
2012-06-01
期刊：
影响因子：
2.1
通讯作者：
Takai, Yoshimi
中科院分区：
文献类型：
--
作者：
Yoshida, Midori;Shimono, Yohei;Takai, Yoshimi
在腭突形成过程中，两侧腭突生长并相互融合，以建立横跨水平腭间充质的连续性。腭架上覆盖着内侧边缘上皮(MEE)，其中大部分顶端细胞是周皮细胞。我们研究了紧密连接(TJ)和粘连连接(AJ)成分以及顶膜标记在腭裂发生中的定位和作用。免疫荧光和免疫电子显微镜分析表明，TJ位于邻近周皮细胞之间的边界，而AJ成分位于MEE中所有上皮细胞之间的边界。具体地说，在邻近周皮细胞之间以及周皮细胞和底层上皮细胞之间观察到典型的AJ，在那里观察到Nextin-1的信号。转化生长因子-β诱导的周皮细胞脱屑降低了残留上皮细胞的极性，从上皮细胞形态的变化以及极性标志物和AJ组分的染色来估计。这些极化程度较低的上皮细胞随后混合在一起，最终至少部分通过细胞凋亡而消失。这些结果表明，覆盖在生长的腭架上的周皮细胞具有真正的TJ，它们的脱屑降低了腭架上皮细胞在腭裂发生中的极性。
In palatogenesis, bilateral palatal shelves grow and fuse with each other to establish mesenchyme continuity across the horizontal palate. The palatal shelves are covered with the medial edge epithelium (MEE) in which most apical cells are periderm cells. We investigated localization and roles of tight junction (TJ) and adherens junction (AJ) components and an apical membrane marker in the MEE in palatogenesis. Immunofluorescence and immunoelectron microscopy analyses revealed that TJs were located at the boundary between neighboring periderm cells, whereas AJ components were localized at the boundary between all epithelial cells in the MEE. Specifically, typical AJs were observed at the boundaries between neighboring periderm cells and between periderm cells and underlying epithelial cells where the signal for nectin-1 was observed. The TGF-beta-induced desquamation of periderm cells reduced the polarity of remaining epithelial cells as estimated by changes of epithelial cell morphology and the staining of the polarity marker and the AJ components. These less polarized epithelial cells then intermingled and finally disappeared at least partly by apoptosis. These results indicate that periderm cells covering growing palatal shelves have bona fide TJs and their desquamation reduces the polarity of palatal shelf epithelial cells in palatogenesis.