Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma
Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma
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DOI：
10.1159/000319897
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Ikeda, Katsuhisa
中科院分区：
文献类型：
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作者：
Haruyama, Takuo;Furukawa, Masayuki;Ikeda, Katsuhisa
目的：本研究旨在阐明人胆脂瘤中 IL-17 的免疫反应性和蛋白水平。程序：通过免疫组织化学检查从 24 名胆脂瘤患者收集的组织标本中 IL-17 和核因子 kappa B 配体受体激活剂 (RANKL) 的表达和定位。通过 CD4 双染色评估 IL-17 的细胞来源。使用酶联免疫吸附测定法测定IL-17蛋白的水平。将骨破坏程度与IL-17免疫反应性进行比较。结果：肉芽组织炎症细胞中检测到IL-17免疫反应性，且IL-17蛋白水平升高。 IL-17 表达的定位与 CD4 阳性淋巴细胞一致。 IL-17 与 RANKL 阳性细胞相关。骨破坏的程度取决于浸润的 IL-17 阳性细胞的数量。与获得性类型相比，在先天性类型中观察到 IL-17 驱动的病理学。结论：本研究提示IL-17与该病的发病机制有关。版权所有 (C) 2010 S. Karger AG，巴塞尔
Purpose: The present study was designed to elucidate the immunoreactivity and protein level of IL-17 in human cholesteatomas. Procedures: The expression and localization of IL-17 and receptor activator of nuclear factor kappa B ligand (RANKL) were examined by immunohistochemistry in tissue specimens collected from 24 patients with cholesteatomas. The cellular sources of IL-17 were assessed by double staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivity. Results: IL-17 immunoreactivity was detected in the inflammatory cells in the granulation tissues and there were increased levels of IL-17 protein. The localization of IL-17 expression coincided with CD4-positive lymphocytes. IL-17 was correlated with the cells positive for RANKL. The degree of bone destruction was dependent on the number of infiltrated IL-17-positive cells. IL-17-driven pathology was observed in the congenital type as compared with the acquired type. Conclusions: The present study suggests that IL-17 is related to the pathogenesis of the disease. Copyright (C) 2010 S. Karger AG, Basel