M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms.
M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms.
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M13 重复探针可检测裸子植物和被子植物中的 DNA 小卫星样序列。
DOI：
10.1073/pnas.85.23.9176
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发表时间：
1988
影响因子：
11.1
通讯作者：
Schaal,BA
中科院分区：
文献类型：
--
作者：
Rogstad,SH;Patton2nd,JC;Schaal,BA
在四足动物基因组中发现了几种由低至中等重复DNA组成的小卫星DNA。虽然给定小卫星的重复寡核苷酸元素（亚重复）实际上是相同的，但不同小卫星之间的亚重复核苷酸组成不同。在一些四足动物中，一些小卫星表现出中等到高水平的限制性内切长度多态性。这种高变量标记为生物学的几个领域的遗传分析提供了强有力的工具。小卫星的应用仅限于四足动物，但在这里，我们证明了一种探针，M13重复探针，以前用于检测人类和牛的小卫星，也揭示了裸子植物和被子植物中携带小卫星的内切酶片段。虽然植物小卫星在个体内似乎是稳定的，但它们在物种内往往以潜在有用的方式变化。这些结果表明，在真核生物中，类似小卫星的家族可能分布在一个广泛的分类范围内，这为小卫星探针在遗传分析中的广泛应用提供了可能性。
Several kinds of minisatellite DNA, all of which are composed of low to moderately repetitive DNA, have been identified in tetrapod genomes. While the repeating oligonucleotide elements (subrepeats) of a given minisatellite are virtually identical, subrepeat nucleotide composition differs between different minisatellites. Several minisatellites have exhibited moderate to high levels of restriction length polymorphism in a number of tetrapods. Such hypervariable markers provide powerful tools for genetic analyses in several fields of biology. Minisatellite applications have been restricted to tetrapods, but here we demonstrate that one probe, the M13 repeat probe previously used to detect minisatellites in humans and bovines, also reveals minisatellite-bearing endonuclease fragments in gymnosperms and angiosperms. While the plant minisatellites appear to be somatically stable within an individual, they often vary within species in potentially useful ways. These results demonstrate that minisatellite-like families may be distributed over a wide taxonomic range in eukaryotes, opening the possibility of a commensurately wide utility of minisatellite probes in genetic analyses.