A Commentary on “Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells” by N. Yamada, T. Okano, H. Sakai, F. Karikusa, Y. Sawasaki, Y. Sakurai (Makromol. Chem., Rapid Commun. 1990, 11, 571–576)
A Commentary on “Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells” by N. Yamada, T. Okano, H. Sakai, F. Karikusa, Y. Sawasaki, Y. Sakurai (Makromol. Chem., Rapid Commun. 1990, 11, 571–576)
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DOI：
10.1002/marc.200500088
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发表时间：
2005-04
影响因子：
4.6
通讯作者：
D. Hutmacher
中科院分区：
文献类型：
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作者：
D. Hutmacher
我很荣幸能为 T. Okano 博士小组的一篇论文撰写评论，该论文在生物材料和组织工程界产生了巨大影响。 1990 年，东京女子医学院生物医学工程研究所的研究小组发表了论文“热响应聚合物表面；控制培养细胞的附着和脱离”。[1]该论文描述了一种收获和扩增细胞的新方法。当利用所谓的“生物纳米界面技术”将温度响应性聚合物，即聚（N-异丙基丙烯酰胺）（PIPAAm）固定到细胞培养塑料板上时，板的表面会响应温度变化而发生可逆的亲水-疏水变化，最低临界溶液温度（LCST）为32±8℃。表面在 32±8C 的温度下变得疏水，使细胞能够附着在表面并生长。 《高分子快速通讯》决定在该期刊创刊25周年之际重印一组过去25年来产生影响的代表性论文。我们如何衡量影响力？ Web of Science 的分析显示，这篇文章被引用了 119 次。搜索门户网站 Medline 和 Science Direct 显示，自 1990 年以来已发表了大量有关 PIPAAm 的论文（图 1）。过去五年中，论文发表量急剧增加，其中许多论文来自东京女子医学院生物医学工程研究所的小组。 Okano 团队及其众多国际合作者最近发表的论文的前 5 名引用次数在 40 到 60 次之间。近几十年来，合成功能聚合物出现了，它们以某种所需的方式响应温度、pH、电场或磁场或一些其他参数的变化。这些聚合物最初被称为“刺激响应”，但“智能”聚合物这个名称是根据它们与生物聚合物的相似性而创造的。研究最多的两种热响应聚合物是聚[(N-异丙基丙烯酰胺)-共-丙烯酰胺]和聚-(NIPAAm)。回顾历史，必须归功于发表第一篇关于 PIPAAm 论文的小组（M.
I am honored to prepare the commentary for a paper of Dr. T. Okano’s group which made a big impact in the biomaterials and tissue engineering community. In 1990 the group at the Institute of Biomedical Engineering in Tokyo Women’s Medical School published their paper ‘‘Thermo-responsive polymeric surfaces; control of attachment and detachment of cultured cells’’.[1] The paper describes a novel methodology to harvest and expand cells. When the temperature responsive polymer, namely poly (N-isopropylacrylamide)(PIPAAm), is fixed to the cell culturing plastic plate utilizing the so called ‘‘bionano-interface technology’’, the surface of the plate changes reversible hydrophilic-hydrophobic in response to temperature change across lowest critical solution temperature (LCST) of 32 8C. The surface becomes hydrophobic over 32 8C which enables cells to attach to the surface and grow. Macromolecular Rapid Communications decided to reprint at the 25th anniversary of the journal a representative set of papers which made an impact over the past 25 years. How do we measure impact? Analysis via the Web of Science shows that the article was cited 119 times. Searching the web portals Medline and Science Direct reveals that a significant number of papers on PIPAAm have been published since 1990 (Figure 1). A steep increase in publication can be observed over the last five years, many of those papers coming out of the group at the Institute of Biomedical Engineering in Tokyo Women’s Medical School. The top five citations of more recent papers from Okano’s group and their numerous international collaborators are in the range of 40 to 60 citations. Recent decades have witnessed the appearance of synthetic functional polymers that respond in some desired way to a change in temperature, pH, electric or magnetic field, or some other parameter. These polymers were originally called ‘stimulus responsive’but the name ‘smart’polymers was coined based on their similarity to biopolymers. The two thermally responsive polymers that are studied most are poly [(N-isopropylacrylamide)-co-acrylamide] and poly-(NIPAAm). Looking into the history, credit has to be given to the group which published the first paper on PIPAAm (M.