The role of microflora in the development of intestinal inflammation:: Acute and chronic colitis induced by dextran sulfate in germ-free and conventionally reared immunocompetent and immunodeficient mice
The role of microflora in the development of intestinal inflammation:: Acute and chronic colitis induced by dextran sulfate in germ-free and conventionally reared immunocompetent and immunodeficient mice
复制标题
DOI：
10.1007/bf02818004
复制
发表时间：
2001-01-01
影响因子：
2.6
通讯作者：
Tlaskalová-Hogenová, H
中科院分区：
文献类型：
--
作者：
Hudcovic, T;Stepánková, R;Tlaskalová-Hogenová, H
常规（CV）免疫缺陷（SCID）小鼠经一周硫酸葡聚糖治疗后，出现急性结肠黏膜结肠炎；无菌（GF） SCID小鼠结肠形态未发生任何变化。硫酸葡聚糖应用于CV免疫活性（BALB/c）小鼠确实诱导了结肠黏膜的实质性变化（4级）；GF BALB/c小鼠仅显示结肠形态轻度改变（1级）。GF SCID小鼠和CV SCID小鼠在第二轮硫酸葡聚糖治疗期间因慢性结肠炎死亡；GF BALB/c小鼠表现为轻度隐窝变形，而CV BALB/c小鼠表现为表面上皮完全缺失（4级），伴有T淋巴细胞和B淋巴细胞浸润。
One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucosa (grade 4); GF BALB/c mice showed mild changes in the colon morphology (grade 1) only. GF SCID mice and CV SCID mice died during the second round of dextran sulfate treatment suffering from chronic colitis; GF BALB/c mice exhibited mild crypt distortion while CV BALB/c mice showed a complete loss of the surface epithelium (grade 4), accompanied by T and B lymphocyte infiltration.