Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in arabidopsis.
Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in arabidopsis.
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DOI：
10.1105/tpc.4.12.1575
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发表时间：
1992-12
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影响因子：
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通讯作者：
Hong Zhang;D. C. Scheirer;William H. Fowle;Howard M. Goodman
中科院分区：
文献类型：
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作者：
Hong Zhang;D. C. Scheirer;William H. Fowle;Howard M. Goodman
分离并表征了编码具有四个锚蛋白重复序列（出现在人蛋白锚蛋白的89K结构域中的33个氨基酸基序）的蛋白质的拟南芥AKR基因。锚蛋白重复序列外的短序列与果蝇肌肉节同源框（msh）基因的蛋白质相似。AKR基因的表达是光依赖性的，并且具有两个或多个拷贝的反义或正义AKR构建体的转基因拟南芥植物以发育调节的方式变得褪绿。褪绿表型遗传传递到下一代，虽然大多数褪绿植物产生少得多的种子。在失绿叶片的质体中发现类囊体膜的减少和基粒的损失，表明反义或正义AKR阻断了叶绿体分化。这项研究表明了锚蛋白重复序列的重要性，不仅在酵母和动物，但在植物中。
The Arabidopsis AKR gene that encodes a protein with four ankyrin repeats (a 33-amino acid motif that appears in the 89K domain of the human protein ankyrin) was isolated and characterized. A short sequence outside the ankyrin repeats is similar to that of the protein of the Drosophila muscle segment homeobox (msh) gene. The expression of the AKR gene is light dependent, and transgenic Arabidopsis plants with two or more copies of an antisense or sense AKR construct became chlorotic in a developmentally regulated manner. The chlorotic phenotype was genetically transmitted to the next generation, although most chlorotic plants produced much less seed. Reduced presence of thylakoid membranes and loss of grana are found in the plastids of chlorotic leaves, indicating that antisense or sense AKR has blocked chloroplast differentiation. This study indicates the importance of ankyrin repeat-containing proteins, not only in yeast and animals, but in plants as well.