Expression of proteasome subunit isoforms during spermatogenesis in Drosophila melanogaster
Expression of proteasome subunit isoforms during spermatogenesis in Drosophila melanogaster
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DOI：
10.1046/j.1365-2583.2002.00374.x
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发表时间：
2002-12
影响因子：
2.6
通讯作者：
J. Ma;E. Katz;J. M. Belote
中科院分区：
文献类型：
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作者：
J. Ma;E. Katz;J. M. Belote
在这项研究中，我们试图确定和表征所有的蛋白酶体基因的果蝇。早期的工作鉴定了两种编码α4-型20 S蛋白酶体亚基亚型的基因，它们仅在雄性生殖系中表达。在这里，我们扩展了这些结果，并表明20 S蛋白酶体亚基中的6个和19 S调节帽亚基中的4个具有编码男性特异性亚型的基因重复。使用GFP标记的报告转基因对其中两个雄性特异性亚基（Prosα 3 T和Prosα 6 T）进行更详细的分析，发现它们主要定位于精子发生后期的细胞核，并存在于成熟的活动精子中。这些结果表明“精子发生特异性”蛋白酶体在精子分化和/或功能中的可能作用。
In this study, we sought to identify and characterize all the proteasome genes of Drosophila melanogaster. Earlier work led to the identification of two genes encoding α4‐type 20S proteasome subunit isoforms that are expressed exclusively in the male germline. Here we extend these results and show that six of the 20S proteasome subunits, and four of the 19S regulatory cap subunits, have gene duplications encoding male‐specific isoforms. More detailed analyses of two of these male‐specific subunits (Prosα3T and Prosα6T), using GFP‐tagged reporter transgenes, revealed that they are predominantly localized to the nucleus at later stages of spermatogenesis and are present there in mature, motile sperm. These results suggest a possible role of a ‘spermatogenesis‐specific’ proteasome in sperm differentiation and/or function.