Characterization of an S-nitroso-N-acetylpenicillamine-based nitric oxide releasing polymer from a translational perspective.
Characterization of an S-nitroso-N-acetylpenicillamine-based nitric oxide releasing polymer from a translational perspective.
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DOI：
10.1080/00914037.2016.1163570
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Handa H
中科院分区：
文献类型：
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作者：
Goudie MJ;Brisbois EJ;Pant J;Thompson A;Potkay JA;Handa H
由于一氧化氮(NO)在调节人体多种生物学功能中的作用，在过去的二十年里，关于不同的NO释放/产生物质的研究已经发表。尽管NO已被证明是一种强大的抗菌和有效的抗血栓药物，但NO释放聚合物(Norel)尚未达到临床应用。虽然增加聚合物中NO供体的浓度是延长NO释放的常用方法，但这不应以牺牲原始材料的机械强度或生物相容性为代价。在本工作中，NO供体分子S-亚硝基青霉胺(SNAP)被引入到Elast-eon E2AS(聚二甲基硅氧烷和聚环氧乙烷的混合软段共聚物)中，不会对基础聚合物的物理和生物属性产生不利影响。在E2AS中加入10wt%的SNAP，极限拉伸强度仅降低20%。SNAP的加入对E2AS聚合物的表面化学和表面粗糙度没有显著影响。紫外线照射、环氧乙烷和过氧化氢蒸汽灭菌技术保留了大约90%的有效SNAP含量，其中这些材料的灭菌在18天内不影响无释放曲线。此外，通过血液相容性和细胞毒性分析，这些Norel材料与宿主组织具有生物相容性。此外，还研究了SNAP在不同储存条件下的稳定性，因为它们与这些材料的平移潜力有关。加入Snap的E2AS在室温下储存超过6个月，保留了其初始SNAP含量的87%。储存的和新鲜的薄膜在18天内表现出相似的NO释放动力学。总而言之，这项研究的结果表明，SNAP掺杂的E2AS聚合物适合于商业生物医学应用，因为已报道的物理和生物特性对商业和临床成功至关重要。
Due to the role of nitric oxide (NO) in regulating a variety of biological functions in humans, numerous studies on different NO releasing/generating materials have been published over the past two decades. Although NO has been demonstrated to be a strong antimicrobial and potent antithrombotic agent, NO-releasing (NOrel) polymers have not reached the clinical setting. While increasing the concentration of the NO donor in the polymer is a common method to prolong the NO-release, this should not be at the cost of mechanical strength or biocompatibility of the original material. In this work, it was shown that the incorporation of S-nitroso-penicillamine (SNAP), an NO donor molecule, into Elast-eon E2As (a copolymer of mixed soft segments of polydimethylsiloxane and poly(hexamethylene oxide)), does not adversely impact the physical and biological attributes of the base polymer. Incorporating 10 wt % of SNAP into E2As reduces the ultimate tensile strength by only 20%. The inclusion of SNAP did not significantly affect the surface chemistry or roughness of E2As polymer. Ultraviolet radiation, ethylene oxide, and hydrogen peroxide vapor sterilization techniques retained approximately 90% of the active SNAP content, where sterilization of these materials did not affect the NO-release profile over an 18 day period. Furthermore, these NOrel materials were shown to be biocompatible with the host tissues as observed through hemocompatibility and cytotoxicity analysis. In addition, the stability of SNAP in E2As was studied under a variety of storage conditions, as they pertain to translational potential of these materials. SNAP-incorporated E2As stored at room temperature for over 6 months retained 87% of its initial SNAP content. Stored and fresh films exhibited similar NO release kinetics over an 18 day period. Combined, the results from this study suggest that SNAP-doped E2As polymer is suitable for commercial biomedical applications due to the reported physical and biological characteristics that are important for commercial and clinical success.