Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 gamma-sigma1 and AP-3 delta-sigma3 hemicomplexes.
Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 gamma-sigma1 and AP-3 delta-sigma3 hemicomplexes.
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DOI：
10.1083/jcb.200307157
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发表时间：
2003-12-22
影响因子：
7.8
通讯作者：
Bonifacino, Juan S
中科院分区：
文献类型：
--
作者：
Janvier, Katy;Kato, Yukio;Boehm, Markus;Rose, Jeremy R;Martina, Jose A;Kim, Bong-Yoon;Venkatesan, Sundararajan;Bonifacino, Juan S
将跨膜蛋白分选至内体和溶酶体由蛋白质的胞质尾部中存在的信号介导。这些信号的子集符合[DE]XXXL[LI]共有基序，并通过与异源四聚体衔接蛋白（AP）复合物的相互作用介导分选。然而，识别这些信号的AP亚基的身份仍然存在争议。我们使用酵母三杂交试验证明，来自人类免疫缺陷病毒阴性因子蛋白和溶酶体整合膜蛋白II的[DE]XXXL[LI]型信号与AP-1的γ和σ1亚基以及AP-3的δ和σ3亚基的组合相互作用，但不与AP-2和AP-4亚基的类似组合相互作用。这些相互作用的序列要求与体外结合整个AP复合物和体内信号功能的序列要求相似。这些观察揭示了涉及AP-1和AP-3的γ/δ和σ亚基的分选信号的新识别模式。
The sorting of transmembrane proteins to endosomes and lysosomes is mediated by signals present in the cytosolic tails of the proteins. A subset of these signals conform to the [DE]XXXL[LI] consensus motif and mediate sorting via interactions with heterotetrameric adaptor protein (AP) complexes. However, the identity of the AP subunits that recognize these signals remains controversial. We have used a yeast three-hybrid assay to demonstrate that [DE]XXXL[LI]-type signals from the human immunodeficiency virus negative factor protein and the lysosomal integral membrane protein II interact with combinations of the γ and σ1 subunits of AP-1 and the δ and σ3 subunits of AP-3, but not the analogous combinations of AP-2 and AP-4 subunits. The sequence requirements for these interactions are similar to those for binding to the whole AP complexes in vitro and for function of the signals in vivo. These observations reveal a novel mode of recognition of sorting signals involving the γ/δ and σ subunits of AP-1 and AP-3.