The MTS assay as an indicator of chemosensitivity/resistance in malignant gynaecological tumours
The MTS assay as an indicator of chemosensitivity/resistance in malignant gynaecological tumours
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DOI：
10.1016/s0361-090x(02)00171-x
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发表时间：
2003-01-01
影响因子：
--
通讯作者：
Bonnar, J
中科院分区：
文献类型：
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作者：
O'Toole, SA;Sheppard, BL;Bonnar, J
本研究的目的是使用（3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑，内盐）（NITS）试验来确定17例子宫内膜和27例宫颈肿瘤对细胞毒性药物的反应。在手术时采集肿瘤样本，并使用外植体技术进行培养。将细胞与化疗药物一起孵育。进行MTS细胞毒性测定以确定对药物的反应并与临床结果回顾性相关。相似分期和级别的肿瘤对药物的反应表现出异质性。90个肿瘤中共有88个（97.8%），包括来自44个卵巢肿瘤样本的早期研究的数据，产生了化疗敏感性数据。共45份标本可评价体外-体内相关性。体外敏感性与30例患者中的26例临床反应相关，体外耐药与15例患者中的14例疾病进展或死亡相关。应进行随机前瞻性试验，以验证化疗敏感性/耐药性试验。(C)2002年国际预防肿瘤学会。由爱思唯尔科技有限公司出版。保留所有权利。
The aim of this study was to use the (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (NITS) assay to determine the response of 17 endometrial and 27 cervical tumours to cytotoxic drugs. Tumour samples were taken at surgery and cultured using the explant technique. Cells were incubated with chemotherapy drugs. The MTS cytotoxicity assay was carried out to ascertain the response to the drugs and correlated retrospectively to the clinical outcome. Tumours of similar stage and grade displayed heterogeneity in their responses to the drugs. A total of 88 of 90 tumours (97.8%), including data from an earlier study of 44 ovarian tumour samples yielded chemosensitivity data. A total of 45 specimens were evaluable for in vitro-in vivo correlations. In vitro sensitivity was associated with clinical response in 26 of 30 patients and in vitro resistance with progressive disease or death in 14 of 15 patients. A randomised prospective trial should be carried out to validate chemosensitivity/resistance testing. (C) 2002 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All rights reserved.