Changes in Plastid DNA Content during the Life Cycle of the Hornwort Anthoceros punctatus L.
Changes in Plastid DNA Content during the Life Cycle of the Hornwort Anthoceros punctatus L.
复制标题
金鱼藻生命周期中质体DNA含量的变化。
作者：
Yoshihiro Izumil;K. Ono
通过对金鱼藻卵细胞、精子细胞、孢子体细胞和配子体细胞质体DNA含量的测定，研究了金鱼藻生活史中质体DNA含量的相对变化。使用落射荧光显微镜和CHIAS观察用DAPI染色的样品。未受精卵细胞的质体DNA含量在受精前和受精后没有变化，这是由于精细胞优先消化细胞器DNA所致。在第2n代，质体通过二次分裂增殖，伴随DNA复制。一般认为质体DNA含量在孢子母细胞中减半，但在孢子萌发前或萌发后立即恢复。在第n代，质体以二分裂方式繁殖，DNA复制，质体DNA含量与第2n代保持相同水平。
The plastid DNA contents of egg, sperm, sporophyte, and gametophyte cells were measured to clarify the relative changes in plastid DNA content during the life cycle of the hornwort Anthoceros punctatus. Samples stained with DAPI were observed using epifluorescence microscopy and CHIAS. The plastid DNA content of unfertilized egg cells did not change before and the fertilization because of preferential digestion of organelle DNA in sperm cells. In the 2n-generation, plastids multiplied by binary fission, with DNA duplication. It is thought that plastid DNA content is halved in spore mother cells, but recovers before, or immediately after, spore germination. In the ngeneration, plastids multiplied by binary fission, with DNA duplication, and the plastid DNA content was maintained at the same level as that of the 2n-generation.