Arabidopsis COBRA-LIKE 10, a GPI-anchored protein, mediates directional growth of pollen tubes
Arabidopsis COBRA-LIKE 10, a GPI-anchored protein, mediates directional growth of pollen tubes
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拟南芥 COBRA-LIKE 10 是一种 GPI 锚定蛋白，介导花粉管的定向生长。
DOI：
10.1111/tpj.12139
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发表时间：
2013-05-01
期刊：
影响因子：
7.2
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Li, Sha;Ge, Fu-Rong;Zhang, Yan
开花植物的成功繁殖需要雌性组织和生长中的花粉管之间的持续交流。雌性细胞通过花粉管胞内活性的动态变化，分泌分子和多肽，作为花粉管快速定向生长的营养物质或引导信号。相对于对雌蜂信号和花粉管胞内活动的广泛关注，雌蜂信号在花粉胞内是如何被感知和解释的却知之甚少。我们在这里展示了COBL10，一种糖基磷脂酰肌醇（GPI）锚定蛋白，是这种花粉管内部机制的一个组成部分。COBL10突变导致雄性配子体不育，原因是花粉管生长减少，雌性传输道的定向感应受损。cobl10花粉管的顶端果胶帽和纤维素微原纤维沉积被破坏。COBL10在花粉管顶端质膜的定位对其功能至关重要，并依赖于适当的GPI加工及其c端疏水残基。gpi锚定蛋白是哺乳动物中广泛存在的细胞传感器，特别是在卵精交流过程中。我们的研究结果表明COBL10对花粉管的定向生长至关重要，这表明它们在植物细胞-细胞通讯中起着关键作用。
Successful reproduction of flowering plants requires constant communication between female tissues and growing pollen tubes. Female cells secrete molecules and peptides as nutrients or guidance cues for fast and directional tube growth, which is executed by dynamic changes of intracellular activities within pollen tubes. Compared with the extensive interest in female cues and intracellular activities of pollen tubes, how female cues are sensed and interpreted intracellularly in pollen is poorly understood. We show here that COBL10, a glycosylphosphatidylinositol (GPI)-anchored protein, is one component of this pollen tube internal machinery. Mutations in COBL10 caused gametophytic male sterility due to reduced pollen tube growth and compromised directional sensing in the female transmitting tract. Deposition of the apical pectin cap and cellulose microfibrils was disrupted in cobl10 pollen tubes. Pollen tube localization of COBL10 at the apical plasma membrane is critical for its function and relies on proper GPI processing and its C-terminal hydrophobic residues. GPI-anchored proteins are widespread cell sensors in mammals, especially during egg-sperm communication. Our results that COBL10 is critical for directional growth of pollen tubes suggest that they play critical roles in cell-cell communications in plants.