Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis.
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis.
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DOI：
10.1371/journal.pntd.0004844
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发表时间：
2016-07
影响因子：
3.8
通讯作者：
Hashiguchi Y
中科院分区：
文献类型：
--
作者：
Kato H;Gomez EA;Martini-Robles L;Muzzio J;Velez L;Calvopiña M;Romero-Alvarez D;Mimori T;Uezato H;Hashiguchi Y
通过使用FTA卡点样本和涂片作为DNA来源，进行了一项全国范围的流行病学研究，以阐明厄瓜多尔皮肤利什曼病（CL）病原种的当前地理分布。假定利什曼原虫在165例样本中收集的CL患者在厄瓜多尔的16个省进行了检查，在物种水平上的细胞色素B基因序列分析的基础上。其中125份标本经鉴定为圭亚那利什曼原虫L.（五）braziliensis、巴西湖蝇L.（五）奈菲湖（五）lainsoni和L.墨西哥利什曼原虫2个优势种，L.（五）guyanensis和L.（五）braziliensis，分别广泛分布于太平洋沿岸亚热带和亚马逊热带地区。最近报道L.（五）naiffi和L.（五）lainsoni和L.（L.）在安第斯高原地区发现了墨西哥。重要的是，本研究表明，L。（五）太平洋沿岸地区巴西丝虫感染正在增加。在厄瓜多尔，利什曼病是该国24个省中的21个省报告的一个主要公共卫生问题，8种利什曼原虫，墨西哥利什曼原虫，L.（L.）amazonensis，亚马逊湖（L.（L.）major-like，L.（Viannia）guyanensis，L.（五）panamensis，L.（五）braziliensis、巴西湖蝇L.（五）naiffi和L.（五）Lainsoni）已被鉴定为人类皮肤利什曼病（CL）和皮肤粘膜利什曼病（MCL）的病原体。CL的致病寄生虫种已被确定为L。（五）guyanensis，L.（五）panamensis，L.（五）braziliensis和L.（L.）amazonensis，L.（L.）mexicana和L.（L.）在安第斯高原地区，L.（五）guyanensis，L.（五）braziliensis、巴西湖蝇L.（五）naiffi和L.（五）亚马逊地区的lainsoni。在本研究中，通过使用FTA卡点样本和涂片作为DNA来源，进行了全国范围的流行病学调查，以阐明厄瓜多尔CL致病种属的当前地理分布。根据细胞色素B基因序列分析，对从厄瓜多尔16个省的CL患者中收集的165份疑似利什曼原虫样本进行了检查。共鉴定出125个样品，其中优势种为L.（五）guyanensis和L.（五）braziliensis，分别广泛分布于太平洋沿岸亚热带和亚马逊热带地区。重要的是，本研究表明，L。（五）太平洋沿岸地区巴西丝虫感染正在增加。
A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) lainsoni, and L. (Leishmania) mexicana. Two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Recently reported L. (V.) naiffi and L. (V.) lainsoni were identified in Amazonian areas, and L. (L.) mexicana was identified in an Andean highland area. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas. In Ecuador, leishmaniasis is a major public health concern reported in 21 of 24 provinces of the country, and eight Leishmania species, Leishmania (Leishmania) mexicana, L. (L.) amazonensis, L. (L.) major-like, L. (Viannia) guyanensis, L. (V.) panamensis, L. (V.) braziliensis, L. (V.) naiffi, and L. (V.) lainsoni, have been identified as causative agents of human cutaneous (CL) and mucocutaneous leishmaniases (MCL). Causative parasite species for CL have been identified as L. (V.) guyanensis, L. (V.) panamensis, L. (V.) braziliensis, and L. (L.) amazonensis in Pacific coast areas, L. (L.) mexicana and L. (L.) major-like in Andean highland areas, and L. (V.) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, and L. (V.) lainsoni in Amazonian areas. In the present study, a countrywide epidemiological survey was performed to elucidate the current geographic distribution of causative species of CL in Ecuador, by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined based on the cytochrome b gene sequence analysis. From these, 125 samples were identified, of which two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas.