Weighted gene co-expression network analysis reveals potential genes involved in early metamorphosis process in sea cucumber Apostichopus japonicus
Weighted gene co-expression network analysis reveals potential genes involved in early metamorphosis process in sea cucumber Apostichopus japonicus
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DOI：
10.1016/j.bbrc.2017.11.154
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Li, Yongxin;Kikuchi, Mani;Wang, Wen
海参是棘皮动物的主要类群之一，在其发育过程中具有非常快速和剧烈的变态过程。然而，这一过程的分子基础在很大程度上仍然未知。本文首次通过RNA测序技术系统研究了日本刺参（Apostichopus cucumber）从受精卵到幼参的16个发育时间点的基因表达谱。基于加权基因共表达网络分析（WGCNA），我们识别了21个模块。其中，MEdarkmagenta基因在黑木耳晚期到长角盲蝽幼虫的早期变态过程中高度表达。此外，基因富集和差异表达基因分析确定了几个基因的模块中，可能发挥关键作用的变态过程。本研究结果不仅为实验研究海参的发育和形态复杂性提供了分子基础，也为提高海参的出苗率奠定了基础。(C)2017爱思唯尔公司All rights reserved.
Sea cucumbers, one main class of Echinoderms, have a very fast and drastic metamorphosis process during their development. However, the molecular basis under this process remains largely unknown. Here we systematically examined the gene expression profiles of Japanese common sea cucumber (Apostichopus japonicus) for the first time by RNA sequencing across 16 developmental time points from fertilized egg to juvenile stage. Based on the weighted gene co-expression network analysis (WGCNA), we identified 21 modules. Among them, MEdarkmagenta was highly expressed and correlated with the early metamorphosis process from late auricularia to doliolaria larva. Furthermore, gene enrichment and differentially expressed gene analysis identified several genes in the module that may play key roles in the metamorphosis process. Our results not only provide a molecular basis for experimentally studying the development and morphological complexity of sea cucumber, but also lay a foundation for improving its emergence rate. (C) 2017 Elsevier Inc. All rights reserved.