Cloning of a novel rat placental prolactin-like protein C-related cDNA.
Cloning of a novel rat placental prolactin-like protein C-related cDNA.
复制标题
新型大鼠胎盘催乳素样蛋白 C 相关 cDNA 的克隆。
DOI：
10.1002/mrd.1080410207
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发表时间：
1995
影响因子：
2.5
通讯作者：
Shiverick,KT
中科院分区：
文献类型：
--
作者：
Conliffe,PR;Simmen,RC;Buhi,WC;Shiverick,KT
催乳素样蛋白 C (PLP-C) 是一种主要的大鼠胎盘蛋白，在妊娠后半期表达，属于生长激素催乳素家族。在这里，我们报告了重叠的大鼠胎盘 cDNA 的分离，该 cDNA 指定了 915 个碱基对的转录物并预测了 205 个氨基酸的翻译产物。全长 cDNA 与报道的 PLP-C 核苷酸序列具有 93% 的同源性，而我们将其命名为 PCRP（催乳素样蛋白 C 相关蛋白）的推定蛋白与 PLP-C 前体蛋白具有 88% 的同源性。 PCRP 缺乏 PLP-C 中存在的信号序列和前 2 个 N 末端半胱氨酸残基。 Northern印迹分析表明PCRP mRNA的基底区特异性表达，在蜕膜和迷路中没有检测到表达。使用 PCRP cDNA 作为探针对大鼠基因组 DNA 进行 Southern 印迹分析，结果显示多个杂交带，表明存在编码催乳素样蛋白的基因家族。使用 PCRP 抗肽抗血清对基底区培养基进行蛋白质免疫印迹分析，结果显示至少有 5 种免疫反应蛋白。妊娠中期后大鼠胎盘中 PLP-C 蛋白家族的存在表明它们在维持妊娠和胎儿发育中的功能意义。 © 1995 Wiley-Liss, Inc.
Prolactin‐like protein C (PLP‐C) is a major rat placental protein which is expressed during the second half of pregnancy and belongs to the growth hormone‐prolactin family. Here we report on the isolation of overlapping rat placental cDNAs which specify a transcript of 915 base pairs and predict a 205‐amino acid translated product. The full‐length cDNA shares 93% homology with the nucleotide sequence reported for PLP‐C, and the putative protein, which we designate PCRP (prolactin‐like protein C‐related protein), exhibits 88% homology with the PLP‐C precursor protein. PCRP lacks the signal sequence and the first 2 N‐terminal cysteine residues present in PLP‐C. Northern blot analysis indicated the basal zone‐specific expression of PCRP mRNA, with no detectable expression in decidua and labyrinth. Southern blot analysis of rat genomic DNA using PCRP cDNA as a probe demonstrated multiple hybridization bands, suggestive of a family of genes encoding prolactin‐like proteins. Western immunoblot analysis of basal zone culture media using a PCRP antipeptide antiserum revealed at least 5 immunoreactive proteins. The existence of a PLP‐C family of proteins in rat placenta after midpregnancy suggests their functional significance in the maintenance of pregnancy and fetal development. © 1995 Wiley‐Liss, Inc.