Bioinformatics analysis of an animal model of diet-induced nonalcoholic fatty liver disease with rapid progression
Bioinformatics analysis of an animal model of diet-induced nonalcoholic fatty liver disease with rapid progression
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DOI：
10.1177/15353702211055099
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发表时间：
2021-11-13
影响因子：
3.2
通讯作者：
Chen, Zhiyun
中科院分区：
文献类型：
--
作者：
Hong, Wei;Zhang, Tingting;Chen, Zhiyun
长爪沙鼠（Meriones unguiculatus）在高脂饮食（HFD）喂养下发生非酒精性脂肪性肝病（NAFLD）。本研究旨在探讨长爪沙鼠的基因表达特征，以更好地了解该动物模型的潜在机制。用普通饲料和高脂饲料喂养长爪沙鼠不同时期。对肝脏mRNA进行高通量测序，并进一步进行生物信息学分析。获得了与炎症、纤维化和HCC相关的8个枢纽基因Cd 44、App、Cdc 42、Cd 68、Cxcr 4、Csf 1 r、Adgre 1和Fermt 3。筛选出4个显著的HCC预后不良独立因素（GPC 1、ARPC 1B、DAB 2和CFL 1）。qRT-PCR结果显示，上述基因在造模过程的不同时期均有高水平表达。本研究结果为长爪沙鼠NAFLD模型的进一步研究提供了有用的信息。
Nonalcoholic fatty liver disease (NAFLD) develops rapidly in high-fat diet (HFD) fed Mongolian gerbil (Meriones unguiculatus). Here, we aim to explore the gene expression characteristics of Mongolian gerbil to better understand the underlying mechanism in this animal model. Mongolian gerbils were fed with normal diet or HFD for different periods. High-throughput sequencing was carried out on the hepatic mRNA and bioinformatics analysis was further performed. Eight hub genes Cd44, App, Cdc42, Cd68, Cxcr4, Csf1r, Adgre1, and Fermt3, which were involved in inflammation, fibrosis, and HCC were obtained. Four significant independent poor prognostic factors for HCC (GPC1, ARPC1B, DAB2, and CFL1) were screened out. qRT-PCR result showed that the above genes expressed high levels in different periods of modeling process. The findings of this study provide useful information for further studies on Mongolian gerbil NAFLD model.