Cph1p negatively regulates MDR1 involved in drug resistance in Candida albicans.
Cph1p negatively regulates MDR1 involved in drug resistance in Candida albicans.
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DOI：
10.1016/j.ijantimicag.2015.01.017
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发表时间：
2015-06
影响因子：
10.8
通讯作者：
H. Lo;Kuo-Yun Tseng;Yeong-Yi Kao;M. Tsao;Hanova Lo;Yun-Liang Yang
中科院分区：
文献类型：
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作者：
H. Lo;Kuo-Yun Tseng;Yeong-Yi Kao;M. Tsao;Hanova Lo;Yun-Liang Yang
白色念珠菌中的cph1/cph1 efg1/efg1双突变体丝状生长缺陷，并且在小鼠模型中无毒力。我们之前报道过 Efg1p 而不是 Cph1p 通过负向调节 ERG3inC 参与耐药性。白色念珠菌。在目前的研究中，我们发现酿酒酵母中CPH1的过度表达会增加抗真菌药物氟康唑的敏感性。此外，inC.在白色念珠菌中，CPH1的无效突变增加了MDR1的表达，并降低了对氟康唑和伏立康唑的敏感性，但对两性霉素B没有敏感性。这些发现表明，尽管Efg1p和Cph1p可能对毒力具有相同的影响，但它们对白色念珠菌的耐药性具有相反的影响。白色念珠菌。
Thecph1/cph1 efg1/efg1double mutant inCandida albicansis defective in filamentous growth and is avirulent in a mouse model. We previously reported that Efg1p but not Cph1p is involved in drug resistance by negatively regulatingERG3inC. albicans. In the current study, we have found that overexpression ofCPH1inSaccharomyces cerevisiaeincreases susceptibility to the antifungal drug fluconazole. Furthermore, inC. albicans, null mutation ofCPH1increased the expression ofMDR1as well as decreased susceptibility to fluconazole and voriconazole but not to amphotericin B. These findings indicate that although Efg1p and Cph1p may have the same effects on virulence, they have opposite effects on drug resistance inC. albicans.