Manufacturing of Proteins and Antibodies: Chapter Downstream Processing Technologies.
Manufacturing of Proteins and Antibodies: Chapter Downstream Processing Technologies.
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蛋白质和抗体的制造：下游加工技术章节。
DOI：
10.1007/10_2016_54
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jean Bender
中科院分区：
文献类型：
--
作者：
R. Turner;A. Joseph;N. Titchener;Jean Bender
细胞收获是从含有靶分子的上清液中分离或保留细胞和细胞碎片。收获方法的选择在很大程度上取决于细胞类型、生物反应器操作模式、工艺规模以及产品和细胞培养液的特性。大多数传统的收获方法使用某种形式的过滤、离心或两者的组合来进行细胞分离和/或保留。过滤方法包括正流深层过滤和切向流微滤。可预测地缩小所选收获方法的能力有助于确保成功生产，并且对于进行小规模表征研究以确认参数目标和范围至关重要。在本章中，我们将介绍离心和深层过滤收获方法，分享收获优化策略，介绍离心缩小模型的最新进展，并回顾替代收获技术。
Cell harvesting is the separation or retention of cells and cellular debris from the supernatant containing the target molecule Selection of harvest method strongly depends on the type of cells, mode of bioreactor operation, process scale, and characteristics of the product and cell culture fluid. Most traditional harvesting methods use some form of filtration, centrifugation, or a combination of both for cell separation and/or retention. Filtration methods include normal flow depth filtration and tangential flow microfiltration. The ability to scale down predictably the selected harvest method helps to ensure successful production and is critical for conducting small-scale characterization studies for confirming parameter targets and ranges. In this chapter we describe centrifugation and depth filtration harvesting methods, share strategies for harvest optimization, present recent developments in centrifugation scale-down models, and review alternative harvesting technologies.