Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration
Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration
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来自人类脱落乳牙的干细胞作为生物牙根再生的替代细胞来源。
DOI：
10.7150/thno.31801
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发表时间：
2019-01-01
期刊：
影响因子：
12.4
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Yang, Xueting;Ma, Yue;Tian, Weidong
干细胞介导的生物工程牙根（bio-root）已被证明是治疗牙齿缺失的一种有前景的工具。我们的前期研究表明，牙囊细胞（DFC）是构建生物牙根的合适种子细胞。然而，DFC只能从未萌牙胚中获得。来自人类脱落乳牙（SHED）的干细胞，通过微创手术更容易获得，可以用作替代种子细胞。本研究比较了SHEDs和DFCs在生物根再生中的成牙特性。然后诱导细胞分泌丰富的细胞外基质（ECM）并形成宏观细胞片。结果：DFCs具有更高的增殖率、更强的成骨和成脂能力，而SHEDs具有更强的迁移能力和更好的神经发生潜能。牙囊细胞片（DFCs）和人脱落乳牙干细胞片（SHEDS）不仅表达ECM蛋白，而且表达成骨和牙源性蛋白。结论：SHEDs和DFCs具有相似的牙源性分化能力，SHEDs/TDM与DFCs/TDM一样，可成功地在体内实现牙周组织的再生，牙周组织由牙周膜纤维、血管和新生牙槽骨组成。
A stem cell-mediated bioengineered tooth root (bio-root) has proven to be a prospective tool for the treatment of tooth loss. As shown in our previous studies, dental follicle cells (DFCs) are suitable seeding cells for the construction of bio-roots. However, the DFCs which can only be obtained from unerupted tooth germ are restricted. Stem cells from human exfoliated deciduous teeth (SHEDs), which are harvested much more easily through a minimally invasive procedure, may be used as an alternative seeding cell. In this case, we compared the odontogenic characteristics of DFCs and SHEDs in bio-root regeneration.Methods: The biological characteristics of SHEDs and DFCs were determined in vitro. The cells were then induced to secrete abundant extracellular matrix (ECM) and form macroscopic cell sheets. We combined the cell sheets with treated dentin matrix (TDM) for subcutaneous transplantation into nude mice and orthotopic jaw bone implantation in Sprague-Dawley rats to further verify their regenerative potential.Results: DFCs exhibited a higher proliferation rate and stronger osteogenesis and adipogenesis capacities, while SHEDs displayed increased migration ability and excellent neurogenic potential. Both dental follicle cell sheets (DFCSs) and sheets of stem cells from human exfoliated deciduous teeth (SHEDSs) expressed not only ECM proteins but also osteogenic and odontogenic proteins. Importantly, similar to DFCSs/TDM, SHEDSs/TDM also successfully achieved the in vivo regeneration of the periodontal tissues, which consist of periodontal ligament fibers, blood vessels and new born alveolar bone.Conclusions: Both SHEDs and DFCs possessed a similar odontogenic differentiation capacity in vivo, and SHEDs were regarded as a prospective seeding cell for use in bio-root regeneration in the future.