Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.
Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.
复制标题
间变性和乳头状人甲状腺癌细胞系体外和体内生长、侵袭和肺部定植的定量和定性差异。
DOI：
10.1007/bf00128960
复制
发表时间：
1996
影响因子：
4
通讯作者：
Zimmer,SG
中科院分区：
文献类型：
--
作者：
Boghaert,ER;Ain,K;Taylor,K;Greenberg,VL;Fowler,C;Zimmer,SG
Invasion and metastasis remain major reasons for failure of anti-cancer therapy. Cell lines derived from human carcinomas are frequently used to investigate the molecular mechanisms that underlie invasion and metastasis. Unfortunately many of these cell lines do not retain the malignant characteristics of their parental tumors. We therefore conducted a series of experimentsin vivoandin vitroto identify which aspects of malignancy of a papillary (NPA'87) and an anaplastic (DR090-1) thyroid carcinoma were consistent with the pathology of the parental tumor types. We evaluated tumor growth, invasion and metastasis of DRO90-1 and NPA'87in vivofollowing inoculation of the tumor cells under the dermis, under the renal capsule and into the lateral tail vein of nude mice. This evaluationin vivoshowed that the anaplastic carcinoma had a faster growth rate compared with the papillary carcinoma. Furthermore, the papillary carcinoma cells could destroy and infiltrate surrounding tissue but were not capable of extravasation and colonization of lung tissue. The anaplastic cells formed lung nodules following injection into the tail vein of nude mice. This lung colonizing capability of DR090-1 correlated with their capacity to secrete an active 62 kDa gelatinase and to migrate through reconstituted basement membranein vitro.
