Regulation of vasoactive intestinal polypeptide release in the suprachiasmatic nucleus circadian clock.
Regulation of vasoactive intestinal polypeptide release in the suprachiasmatic nucleus circadian clock.
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DOI：
10.1097/wnr.0b013e32833fcba4
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发表时间：
2010-11-17
期刊：
影响因子：
1.7
通讯作者：
Glass JD
中科院分区：
文献类型：
--
作者：
Francl JM;Kaur G;Glass JD
哺乳动物视交叉上核（SCN）生物钟的时间是由视网膜的光输入调节的。视网膜受体单位通过释放血管活性肠多肽（VIP）来调控SCN起搏器细胞的节律性。然而，这一过程的潜在性质是推测性的，因为体内SCN VIP释放从未被测量过。在这里，SCN微透析被用于研究VIP的调节机制。在LD 14:10下的仓鼠在中午附近突触VIP释放达到每日峰值。在持续的黑暗中，这种输出是不规律的。光照和谷氨酸能激动剂NMDA在夜间刺激VIP释放，而5-HT1A，7激动剂8-OH-DPAT在中午抑制VIP释放。因此，SCN VIP活性受到光输入的刺激，并受到血清素的抑制。
Timing of the mammalian circadian clock of the suprachiasmatic nucleus (SCN) is regulated by photic input from the retina. Retinorecipient units entrain rhythmicity of SCN pacemaker cells in part through their release of vasoactive intestinal polypeptide (VIP). The underlying nature of this process is conjectural however, as in vivo SCN VIP release has never been measured. Here, SCN microdialysis was used to investigate mechanisms regulating VIP. Hamsters under LD 14:10 exhibited a daily peak in synaptic VIP release near midday. Under constant darkness, this output was arrhythmic. Light and the glutamatergic agonist NMDA stimulated VIP release at night, while the 5-HT1A,7 agonist, 8-OH-DPAT, suppressed release at midday. Thus, SCN VIP activity is stimulated by photic input and inhibited by serotonin.