Studies of human cord blood and adult lymphocyte interactions with in vitro immunoglobulin production.
Studies of human cord blood and adult lymphocyte interactions with in vitro immunoglobulin production.
复制标题
人脐带血和成人淋巴细胞与体外免疫球蛋白生产相互作用的研究。
DOI：
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发表时间：
1979
影响因子：
15.9
通讯作者：
R. Williams
中科院分区：
文献类型：
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作者：
T. Morito;A. Bankhurst;R. Williams
尽管存在具有表面免疫球蛋白（IG）的循环淋巴细胞，但新生儿不能产生正常量的免疫球蛋白。本研究旨在研究新生儿IG合成受损的细胞基础。采用体外IgG和IgM合成测定，其测量美洲商陆有丝分裂原刺激的脐带血和/或成人外周单核细胞（MNC）的上清液中存在的IG。结果如下：（a）向成人MNC中加入脐带血MNC可抑制正常IgG和IgM的产生;（B）向脐带骨髓来源的（B）细胞中加入成人胸腺来源的（T）细胞悬液并不增加Ig的产生;（c）向成人B细胞中加入脐带T细胞并不增加正常IG的产生，但显著抑制IgM和IgG的合成;和（d）用2，000拉德照射脐带T细胞消除了脐带T细胞对成体MNC的抑制作用。在存在10 μ M氢化可的松的情况下，也观察到脐带MNC所施加的抑制作用的类似逆转。新生儿不能产生正常量的IG似乎是由于B细胞缺陷和类固醇敏感性和放射敏感性抑制性脐带T细胞的存在。
Newborns are unable to produce normal amounts of immunoglobulin despite the presence of circulating lymphocytes with surface immunoglobin (Ig). This study was designed to examine the cellular basis of such impaired Ig synthesis in the newborn infant. An in vitro assay for IgG and IgM synthesis was employed which measured the Ig present in the supernates of pokeweed mitogen-stimulated cord blood and/or adult peripheral mononuclear cells (MNC). Results were as follows: (a) the addition of cord blood MNC to adult MNC suppressed both normal IgG and IgM production; (b) addition of a suspension of adult thymus-derived (T) cells to cord bone marrow-derived (B) cells did not enhance the production of Ig; (c) the addition of cord T cells to adult B cells did not enhance normal Ig production but did significantly depress IgM and IgG synthesis; and (d) irradiation of cord T cells with 2,000 rads removed the suppressive effect of cord T cells on adult MNC. A similar reversal of the suppressive effect exerted by cord MNC was also seen in the presence of 10 microM of hydrocortisone. It appears that the inability of newborn infants to make normal amounts of Ig is a result of a combined B-cell defect and the presence of a steroid-sensitive and radiosensitive suppressor cord T cell.