SPLENIC DEPENDENCE OF THE ANTIBODY-RESPONSE TO THYMUS-INDEPENDENT (TI-2) ANTIGENS
SPLENIC DEPENDENCE OF THE ANTIBODY-RESPONSE TO THYMUS-INDEPENDENT (TI-2) ANTIGENS
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DOI：
10.1002/eji.1830150516
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
HUMPHREY, JH
中科院分区：
文献类型：
--
作者：
AMLOT, PL;GRENNAN, D;HUMPHREY, JH
脾切除术后很长时间，对T非依赖性（TI）-2抗原[2，4-二硝基苯基-赖氨酸-Ficoll（DNP-FIC）和DNP-羟乙基淀粉（HES）]的小鼠抗体应答受损;对TI-1 [三硝基苯基脂多糖（TNP-LPS）]和胸腺依赖性[TNP-keyhole limpet血蓝蛋白（KLH）]抗原的应答基本不受影响。对这些TI-2抗原的抗体应答仅针对缀合的表位[DNP-、异硫氰酸荧光素（FITC）-或异硫氰酸四甲基罗丹明（TRITC）-Ficoll或-HES]。Ficoll和HES的荧光缀合物选择性定位于脾边缘区巨噬细胞。这种定位不受750 cGy [厘戈瑞]的X射线照射的影响，但对TI-2抗原的抗体反应被废除了14天。脾细胞的管理恢复这些TI-2抗原的抗体反应，否则完整的照射小鼠，但如果他们已经被脾切除。对TI-2抗原的抗体应答显然取决于脾环境中B细胞的刺激。这可能涉及边缘区巨噬细胞的抗原呈递。
The murine antibody response to T-independent (TI)-2 antigens [2,4-dinitrophenyl-Lys-Ficoll (DNP-FIC) and DNP-hydroxyethyl starch (HES)]) was impaired long after splenectomy; responses to TI-1 [trinitrophenylated lipopolysaccharide (TNP-LPS)] and thymus-dependent [TNP-keyhole limpet hemocyanin (KLH)] antigens were largely unaffected. The antibody response to these TI-2 antigens was exclusively against the conjugated epitopes [DNP-, fluorescein isothiocyanate (FITC)- or tetramethylrhodamine isothiocyanate (TRITC)-Ficoll or -HES]. Fluorescent conjugates of Ficoll and HES localize selectively to the splenic marginal zone macrophages. This localization was not affected by 750 cGy [centigray] of X-irradiation, but the antibody response to the TI-2 antigens was abrogated for 14 days. Administration of spleen cells restored the antibody response to these TI-2 antigens in otherwise intact irradiated mice but not if they had been splenectomized. The antibody response to TI-2 antigens apparently depends upon stimulation of B cells in a splenic environment. This probably involves antigen presentation by marginal zone macrophages.