Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts
Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts
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DOI：
10.1111/j.1471-4159.2008.05523.x
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发表时间：
2008-08-01
影响因子：
4.7
通讯作者：
Bermudez-Rattoni, Federico
中科院分区：
文献类型：
--
作者：
Delint-Ramirez, Ilse;Salcedo-Tello, Pamela;Bermudez-Rattoni, Federico
海马和岛叶皮质中的 NMDA 受体 (NMDAR) 激活对于空间记忆的形成是必要的。最近的研究表明，NMDAR 定位于脂筏增强了其信号传导，因为磷酸化其亚基的激酶在脂筏膜微域中存在较大比例。我们试图确定 NMDAR 易位到突触脂筏的可能性发生在记忆形成等可塑过程中。我们的结果表明，水迷宫训练诱导 NMDAR 亚基（NR1、NR2A、NR2B）和 PSD-95 快速募集到突触脂筏，并降低突触后密度，加上大鼠岛叶皮层酪氨酸 1472 处 NR2B 磷酸化增加。在海马体中，空间训练诱导 NR1 和 NR2A 亚基选择性易位至脂筏。这些结果表明，在空间记忆形成过程中，NMDAR 从突触后膜（非筏 PSD）的可溶部分易位到突触脂筏。 NMDA 受体和其他蛋白质向脂筏的募集可能是提高突触可塑性过程中突触传递效率的重要机制。
NMDA receptors (NMDARs) activation in the hippocampus and insular cortex is necessary for spatial memory formation. Recent studies suggest that localization of NMDARs to lipid rafts enhance their signalization, since the kinases that phosphorylate its subunits are present in larger proportion in lipid raft membrane microdomains. We sought to determine the possibility that NMDAR translocation to synaptic lipid rafts occurs during plasticity processes such as memory formation. Our results show that water maze training induces a rapid recruitment of NMDAR subunits (NR1, NR2A, NR2B) and PSD-95 to synaptic lipid rafts and decrease in the post-synaptic density plus an increase of NR2B phosphorylation at tyrosine 1472 in the rat insular cortex. In the hippocampus, spatial training induces selective translocation of NR1 and NR2A subunits to lipid rafts. These results suggest that NMDARs translocate from the soluble fraction of post-synaptic membrane (non-raft PSD) to synaptic lipid raft during spatial memory formation. The recruitment of NMDA receptors and other proteins to lipid rafts could be an important mechanism for increasing the efficiency of synaptic transmission during synaptic plasticity process.