Isolation of human and porcine islets of Langerhans and islet transplantation in pigs.
Isolation of human and porcine islets of Langerhans and islet transplantation in pigs.
复制标题
人和猪胰岛的分离以及猪胰岛移植。
DOI：
10.1016/0022-4804(74)90017-1
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. Najarian
中科院分区：
文献类型：
--
作者：
D. Sutherland;Michael W. Steffes;G.Eric Bauer;Darlene McManus;Bran D. Noe;J. Najarian
采用改良的胶原酶消化法，Ficoll梯度分离技术，从脑死亡的新鲜人胰腺中分离胰岛。整个胰腺和分离材料之间的组织胰岛素/淀粉酶比率的比较显示从腺泡组织中显著纯化胰岛。此外，通过这种技术分离的胰岛，当incubatedin体外，纳入放射性标记的氨基酸前体酸-醇-可溶性胰岛蛋白，从而表明他们的活力和蛋白质激素合成capability.Pig胰腺通过类似的技术处理，和分离的材料移植到猪与糖尿病引起的全胰腺切除术。接受胰岛移植的11头胰腺切除猪的平均存活时间为15.3天，而13头胰腺切除对照猪的平均存活时间为6.0天。对照猪在胰腺切除术后2天没有检测到循环胰岛素。接受胰岛移植的切除胰腺的猪，尽管仍有高血糖，但移植后25天内胰岛素水平在5 - 14 μU/ml之间。
Using a modification of the collagenase digestion, Ficoll gradient separation technique, we isolated the islets of Langerhans from fresh human pancreata removed from braindead cadavers. Comparison of the tissue insulin/amylase ratios between whole pancreas and isolated material revealed a significant purification of islet from acinar tissue. Also, islets isolated by this technique, when incubatedin vitro, incorporated radiolabeled amino acid precursors into acid-alcohol-soluble islet proteins, thus indicating their viability and protein hormonal synthesis capabilities.Pig pancreata were processed by a similar technique, and the isolated material was transplanted to pigs with diabetes induced by total pancreatectomy. The mean survival time of the 11 pancreatectomized pigs that received an islet transplant was 15.3 days, while 13 pancreatectomized control pigs had a mean survival time of 6.0 days. Control pigs had no detectable circulating insulin 2 days after pancreatectomy. The pancreatectomized pigs that received an islet transplant, although remaining hyperglycemic, had insulin levels ranging from 5 to 14 μU/ml for up to 25 days after transplantation.The present studies demonstrate the feasibility of an approach designed for our ultimate goal—the large scale isolation and transplantation of islets in man.